{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,24]],"date-time":"2025-10-24T12:48:27Z","timestamp":1761310107707},"reference-count":16,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2003,6,16]],"date-time":"2003-06-16T00:00:00Z","timestamp":1055721600000},"content-version":"vor","delay-in-days":15,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Phytotherapy Research"],"published-print":{"date-parts":[[2003,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The hepatoprotective effect of the ethanol extract (AvEE) and the main \ufb02avonoid compound 4\u2032\u2010methoxy\u20105,7\u2010dihydroxy\ufb02avone 6\u2010C\u2010<jats:italic>\u03b2<\/jats:italic>\u2010glucopyranoside (isocytisoside, ISOC) from the leaves and stems of <jats:italic>Aquilegia vulgaris<\/jats:italic> L. were studied using the CCl<jats:sub>4<\/jats:sub>\u2010induced hepatotoxicity test.<\/jats:p><jats:p>The acute toxicity test in mice showed that AvEE can be classi\ufb01ed as nontoxic since a dose of 3000 mg\/ kg did not cause mortality.<\/jats:p><jats:p>The barbiturate\u2010induced sleeping time prolonged by CCl<jats:sub>4<\/jats:sub> administration to mice was signi\ufb01cantly reduced after AvEE treatment proving the protective effect of the extract on microsomal drug\u2010metabolizing enzymes.<\/jats:p><jats:p>AvEE and ISOC administered to rats 48 h, 24 h and 2 h before, and 6 h after CCl<jats:sub>4<\/jats:sub> intoxication caused a signi\ufb01cant decrease in the CCl<jats:sub>4<\/jats:sub>\u2010induced elevation of hepatic enzymes activity in serum, i.e. sorbitol dehydrogenase (SDH), glutamate oxaloacetate and glutamate pyruvate transaminases (GOT, GPT).<\/jats:p><jats:p>Both substances induced CCl<jats:sub>4<\/jats:sub>\u2010diminished erythrocyte superoxide dismutase (SOD) and reduced the activities of glutathione peroxidase (GPx) and glutathione reductase (GR) preliminarily enhanced by CCl<jats:sub>4<\/jats:sub>.<\/jats:p><jats:p>The hepatoprotective properties of AvEE and ISOC were con\ufb01rmed by pathomorphological examination of the liver. 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