{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T13:28:18Z","timestamp":1762003698962},"reference-count":27,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2004,5,26]],"date-time":"2004-05-26T00:00:00Z","timestamp":1085529600000},"content-version":"vor","delay-in-days":25,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Phytotherapy Research"],"published-print":{"date-parts":[[2004,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The crude methanol extract of the leaf of <jats:italic>Allium ascalonicum<\/jats:italic> was screened <jats:italic>in vitro<\/jats:italic> against \ufb01ve strains of <jats:italic>Helicobacter pylori<\/jats:italic> (Hp) (ATCC 24376, UCH 97001, UCH 97009, UCH 98026 and UCH 99039) for antibacterial activity by the agar diffusion method in Mueller\u2010Hinton agar supplemented with de\ufb01brinated horse blood. All the strains were inhibited by the extract to varying degrees. The minimum inhibitory concentrations (MICs) of the extract against all the tested strains ranged from 6.25 to 12.5 mg\/mL. The effects of increasing concentrations of the extract on the urease activity of three of the <jats:italic>Helicobacter pylori<\/jats:italic> strains were investigated further. The results showed that increasing the concentration of the extract decreased the urease activity of all the strains tested. Phytochemical screening of the plant showed that it contains alkaloids, cardiac glycosides and saponins. The anti\u2010Hp activity observed is discussed in relation to the chemical constituents reportedly isolated from these plants and their traditional uses. The result of this work suggests that <jats:italic>Allium ascalonicum<\/jats:italic> has some therapeutic potential against <jats:italic>Helicobacter pylori<\/jats:italic> infection, which could be explored for patients with gastroduodenal disorders. Copyright \u00a9 2004 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/ptr.1265","type":"journal-article","created":{"date-parts":[[2004,5,26]],"date-time":"2004-05-26T17:01:07Z","timestamp":1085590867000},"page":"358-361","source":"Crossref","is-referenced-by-count":32,"title":["<i>In vitro<\/i> anti\u2010<i>Helicobacter pylori<\/i> potential of methanol extract of <i>Allium ascalonicum<\/i> Linn. (Liliaceae) leaf: susceptibility and effect on urease activity"],"prefix":"10.1002","volume":"18","author":[{"given":"Bolanle A.","family":"Adeniyi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Festus M.","family":"Anyiam","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,5,26]]},"reference":[{"key":"e_1_2_1_2_1","first-page":"221","article-title":"Antimicrobial potential of Diospyros mespiliformis (Ebeneceae)","volume":"25","author":"Adeniyi BA","year":"1996","journal-title":"Afr J Med Med Sci"},{"key":"e_1_2_1_3_1","first-page":"157","volume-title":"The State of Medicinal Plants Research in Nigeria","author":"Akinniyi JA","year":"1986"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1999.tb13447.x"},{"key":"e_1_2_1_5_1","first-page":"634","article-title":"Therapeutic values of onion (Allium cepa L.) and garlic (Allium sativum)","volume":"34","author":"Augusti KT","year":"1996","journal-title":"Indian J Exp Biol"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/clind\/15.3.386"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.3.1.1"},{"key":"e_1_2_1_8_1","first-page":"484","volume-title":"The Useful Plants of the West Africa","author":"Dalziel JM","year":"1937"},{"key":"e_1_2_1_9_1","first-page":"720","article-title":"Helicobacter pylori eradication","volume":"257","author":"David S","year":"1996","journal-title":"Pharm J"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1472-765X.1991.tb00496.x"},{"key":"e_1_2_1_11_1","first-page":"15","volume-title":"Ethnomedical Uses of Plants in Nigeria","author":"Gills LS","year":"1992"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.39.4.353"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0016-5085(89)80057-5"},{"key":"e_1_2_1_14_1","first-page":"327","article-title":"Rapid screening for urease inhibitors","volume":"19","author":"Hamilton\u2010Miller JMT","year":"1979","journal-title":"Invest Urol"},{"key":"e_1_2_1_15_1","first-page":"228","volume-title":"Phytochemical Methods; Guide to Modern Techniques of Plant Analysis","author":"Harbone JB","year":"1991"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(86)92561-4"},{"key":"e_1_2_1_17_1","first-page":"156","article-title":"Pharmacological therapy of Helicobacter pylori infection","volume":"8","author":"Hoffman JS","year":"1997","journal-title":"Semin Gastrointest Dis"},{"key":"e_1_2_1_18_1","doi-asserted-by":"crossref","first-page":"992","DOI":"10.1128\/iai.58.4.992-998.1990","article-title":"Purification and N\u2010terminal analysis of urease from Helicobacter pylori.","volume":"58","author":"Hu LT","year":"1990","journal-title":"Infect Immun"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-1746.1997.tb00491.x"},{"key":"e_1_2_1_20_1","first-page":"57","article-title":"Cultivated and semi\u2010cultivated leafy vegetables of West African","volume":"2","author":"Irvine FR","year":"1956","journal-title":"Material Veg"},{"key":"e_1_2_1_21_1","first-page":"59","volume-title":"Woody Plants of Ghana","author":"Irvine FR","year":"1961"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-8741(94)90094-9"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.3109\/00365529109103999"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/153.4.650"},{"key":"e_1_2_1_25_1","first-page":"1314","article-title":"Unidentified curved bacilli in the stomach of patients with gastritis and peptic ulceration","author":"Marshall BJ","year":"1984","journal-title":"Lancet"},{"key":"e_1_2_1_26_1","doi-asserted-by":"crossref","first-page":"831","DOI":"10.1128\/jcm.26.5.831-836.1988","article-title":"Characterization of urease from Campylobacter pylori.","volume":"26","author":"Mobley HLT","year":"1988","journal-title":"J Clin Microbiol"},{"key":"e_1_2_1_27_1","unstructured":"1995 National Committee for Clinical Laboratory Standards Villanova National Committee for Clinical Laboratory Standards Reference Method for broth dilution antifungal susceptibility testing of yeasts. 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