{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,12]],"date-time":"2025-11-12T20:27:58Z","timestamp":1762979278023},"reference-count":22,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2003,10,27]],"date-time":"2003-10-27T00:00:00Z","timestamp":1067212800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Phytotherapy Research"],"published-print":{"date-parts":[[2003,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Catechins are polyphenols with antioxidant activity. The fruit <jats:italic>Cocos nucifera<\/jats:italic> (Palmae) has a \ufb01ber husk rich in catechins and the local population of northeast Brazil uses it as a medicine against various diseases. An anti\u2010bacterial and anti\u2010viral activity has been already observed using this substance. Plant extracts, from other sources, rich in catechins are inhibitory to tumour cells, suppressing their proliferation. The aim of the present work was to verify if catechins isolated from <jats:italic>Cocos nucifera<\/jats:italic> were capable of inhibiting cell proliferation. An extract obtained from <jats:italic>Cocos nucifera<\/jats:italic> was puri\ufb01ed through adsorption chromatography using the resin XAD\u20102. The puri\ufb01ed material was used in cultures of an erythroleukaemia cell line (K562) and on normal human peripheral blood lymphocytes. Cell viability was assessed using MTT. Cellular proliferation was measured by [<jats:sup>3<\/jats:sup>H]\u2010thymidine incorporation and cell cycle analysis in a \ufb02ow cytometer. A dose\u2010dependent inhibitory effect was observed on tumour cells and on lymphocytes activated by phytohemaglutinin (PHA) or phorbol ester. For PHA this effect was irreversible being already established on the \ufb01rst four hours of culture. Copyright \u00a9 2003 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/ptr.1297","type":"journal-article","created":{"date-parts":[[2003,10,28]],"date-time":"2003-10-28T08:01:35Z","timestamp":1067328095000},"page":"1054-1058","source":"Crossref","is-referenced-by-count":36,"title":["The effect of a catechin\u2010rich extract of <i>Cocos nucifera<\/i> on lymphocytes proliferation"],"prefix":"10.1002","volume":"17","author":[{"given":"Clarice","family":"Kirszberg","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniele","family":"Esquenazi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Celuta Sales","family":"Alviano","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vivian M.","family":"Rumjanek","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2003,10,27]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/89.24.1881"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/19.12.2201"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3835(98)00108-6"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0923-2508(02)01377-3"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0027-5107(97)00310-2"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0091-7435(92)90057-O"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0192-0561(92)90011-9"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-2006-958030"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/19.3.419"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/20.4.733"},{"key":"e_1_2_1_12_1","doi-asserted-by":"crossref","first-page":"619","DOI":"10.1007\/978-1-4899-2911-2_15","volume-title":"The Flavonoids \u2013 Advances in Research Since 1986","author":"Middleton E","year":"1993"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(83)90303-4"},{"key":"e_1_2_1_14_1","first-page":"65","article-title":"Inhibition of influenza virus infection by tea","volume":"9","author":"Nakayama M","year":"1990","journal-title":"Lett Appl Microbiol"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3835(98)00084-6"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0041-0101(00)00154-9"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1472-765X.1989.tb00255.x"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-1218(89)90120-1"},{"issue":"8","key":"e_1_2_1_19_1","doi-asserted-by":"crossref","first-page":"1662","DOI":"10.1002\/(SICI)1097-0142(19960415)77:8<1662::AID-CNCR36>3.0.CO;2-W","article-title":"Inhibitor effects and toxicity of green tea polyphenols for gastrointestinal carcinogenesis","volume":"77","author":"Yamane T","year":"1996","journal-title":"Cancer"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/85.13.1038"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/19.4.611"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/0031-9422(90)80121-V"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0192-0561(97)00074-X"}],"container-title":["Phytotherapy Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fptr.1297","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ptr.1297","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,17]],"date-time":"2023-11-17T18:42:41Z","timestamp":1700246561000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ptr.1297"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,10,27]]},"references-count":22,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2003,11]]}},"alternative-id":["10.1002\/ptr.1297"],"URL":"https:\/\/doi.org\/10.1002\/ptr.1297","archive":["Portico"],"relation":{},"ISSN":["0951-418X","1099-1573"],"issn-type":[{"value":"0951-418X","type":"print"},{"value":"1099-1573","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,10,27]]}}}