{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T00:25:33Z","timestamp":1791073533836,"version":"4.1.0"},"reference-count":7,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2003,12,5]],"date-time":"2003-12-05T00:00:00Z","timestamp":1070582400000},"content-version":"vor","delay-in-days":4,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Phytotherapy Research"],"published-print":{"date-parts":[[2003,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The anti\u2010sickling activities of the extracts of the roots of a plant <jats:italic>Cissus populnea<\/jats:italic> L. (CPK) (a major constituent of a herbal formula Ajawaron HF used in the management of sickle cell disease in south\u2010west Nigeria) has been examined. Phytochemical examination of the extract showed the presence of anthraquinone derivatives, steroidal glycosides and cardiac glycosides. Alkaloids and tannins were completely absent in the CPK extracts. Evaluation of the anti\u2010sickling activity involved the use of both positive (<jats:italic>p<\/jats:italic>\u2010hydroxybenzoic acid, 5 \u00b5g\/mL) and negative control (normal saline) for each set of experiments aimed at the inhibition of sodium metabisulphite\u2010induced sickling of the HbSS red blood cells obtained from con\ufb01rmed non\u2010crisis state sickle\u2010cell patients. The chloroform and water partitioned fractions of the cold methanol extracts of CPK exhibited a 62.2% and 52.9% inhibition of sickling, respectively, at 180 min. The herbal formula (HF) aqueous extract showed the highest anti\u2010sickling activity on a weight by weight basis of all the extracts and fractions tested, giving a 71.4% inhibition of sickling at the end of 180 min incubation when compared with the normal saline control. The maximum percentage inhibition of sickling exhibited by the <jats:italic>p<\/jats:italic>\u2010hydroxybenzoic acid control was 46.0% at 90 min incubation. Copyright \u00a9 2003 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/ptr.1323","type":"journal-article","created":{"date-parts":[[2003,12,8]],"date-time":"2003-12-08T08:01:53Z","timestamp":1070870513000},"page":"1173-1176","source":"Crossref","is-referenced-by-count":36,"title":["Anti\u2010sickling potential of a Nigerian herbal formula (ajawaron HF) and the major plant component (<i>Cissus populnea<\/i> L. CPK)"],"prefix":"10.1002","volume":"17","author":[{"given":"J. O.","family":"Moody","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"O. O.","family":"Ojo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"O. O.","family":"Omotade","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. A.","family":"Adeyemo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P. E.","family":"Olumese","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"O. O.","family":"Ogundipe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2003,12,5]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-2006-961475"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1097\/00000441-197305000-00003"},{"key":"e_1_2_1_4_1","first-page":"157","article-title":"Clinical evaluation of cyanate in sickle cell anaemia","volume":"9","author":"Harkness DR","year":"1975","journal-title":"Prog Haematology"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(72)90359-5"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1159\/000208177"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-2007-969243"},{"key":"e_1_2_1_8_1","volume-title":"Standardization and Utility of Herbal Medicines","author":"Wambebe CON","year":"1998"}],"container-title":["Phytotherapy Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fptr.1323","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ptr.1323","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,17]],"date-time":"2023-11-17T22:25:34Z","timestamp":1700259934000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ptr.1323"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,12]]},"references-count":7,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2003,12]]}},"alternative-id":["10.1002\/ptr.1323"],"URL":"https:\/\/doi.org\/10.1002\/ptr.1323","archive":["Portico"],"relation":{},"ISSN":["0951-418X","1099-1573"],"issn-type":[{"value":"0951-418X","type":"print"},{"value":"1099-1573","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,12]]}}}