{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T21:06:13Z","timestamp":1790111173252,"version":"4.0.1"},"reference-count":18,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2004,5,18]],"date-time":"2004-05-18T00:00:00Z","timestamp":1084838400000},"content-version":"vor","delay-in-days":47,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Phytotherapy Research"],"published-print":{"date-parts":[[2004,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Betaine, coumarin, hesperidin and kaempferol are the components derived from <jats:italic>Lycium chinense<\/jats:italic>, <jats:italic>Angelicae decursiva<\/jats:italic>, <jats:italic>Poncirus trifoliata<\/jats:italic> and <jats:italic>Polygonatum odoratum<\/jats:italic>, respectively. These plants have been used for the treatment of respiratory diseases in oriental medicine and their respective components were reported to have various biological effects. In this study, we investigated whether these natural products affect mucin release from cultured hamster tracheal surface epithelial cells and compared the possible activities of these agents with the inhibitory action on mucin release by poly\u2010L\u2010lysine and the stimulatory action by adenosine triphosphate. Con\ufb02uent primary hamster tracheal surface epithelial cells were metabolically radiolabeled using <jats:sup>3<\/jats:sup>H\u2010glucosamine for 24 h and treated for 30 min in the presence of varying concentrations of each agent to assess the effects on <jats:sup>3<\/jats:sup>H\u2010mucin release. The results were as follows: (i) Coumarin and kaempferol did not affect mucin release signi\ufb01cantly; (ii) Betaine and hesperidin increased mucin release at the highest concentration; (iii) Poly\u2010L\u2010lysine inhibited and adenosine triphosphate increased mucin release. We conclude that betaine and hesperidin can increase mucin release by direct acting on airway mucin\u2010secreting cells and suggest these agents be further studied for the possible use as mild expectorants during the treatment of chronic airway diseases. Copyright \u00a9 2004 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/ptr.1433","type":"journal-article","created":{"date-parts":[[2004,5,18]],"date-time":"2004-05-18T15:02:36Z","timestamp":1084892556000},"page":"301-305","source":"Crossref","is-referenced-by-count":28,"title":["Effects of betaine, coumarin and flavonoids on mucin release from cultured hamster tracheal surface epithelial cells"],"prefix":"10.1002","volume":"18","author":[{"given":"Choong Jae","family":"Lee","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jae Heun","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeong Ho","family":"Seok","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gang Min","family":"Hur","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ji sun","family":"Park","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sohyun","family":"Bae","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jong Ho","family":"Lim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang Chun","family":"Park","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2004,5,18]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-2006-960838"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0091-6749(83)90479-7"},{"issue":"7","key":"e_1_2_1_4_1","first-page":"283","article-title":"Protective antiallergic effects of a new coumarin compound in experimental asthma","volume":"17","author":"Gonsior E","year":"1979","journal-title":"Int J Clin Pharmacol Biopharm"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.97.10112644"},{"key":"e_1_2_1_6_1","doi-asserted-by":"crossref","first-page":"4021","DOI":"10.1016\/S0021-9258(18)89225-0","article-title":"Biochemical characterization of mucous glycoproteins synthesized and secreted by hamster tracheal epithelial cells in primary culture.","volume":"260","author":"Kim KC","year":"1985","journal-title":"J Biol Chem"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.84.24.9304"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.1999.277.4.L811"},{"key":"e_1_2_1_9_1","first-page":"L902","article-title":"Flavonoids stimulate Cl conductance of human airway epithelium in vitro and in vivo","volume":"275","author":"llek B","year":"1998","journal-title":"Am J Physiol"},{"key":"e_1_2_1_10_1","volume-title":"Drug Actions","author":"Mutschler E","year":"1995"},{"key":"e_1_2_1_11_1","volume-title":"Textbook of Pulmonary Disease","author":"Newhouse MT","year":"1983"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/1520-6866(2001)21:2<181::AID-TCM7>3.0.CO;2-2"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.3109\/01902149009087875"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.3109\/01902149309064354"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2249.1991.tb08167.x"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.3177\/jnsv.47.270"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1099-1573(199911)13:7<601::AID-PTR550>3.0.CO;2-O"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1177\/36.2.3335774"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-2952(02)01445-4"}],"container-title":["Phytotherapy Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fptr.1433","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ptr.1433","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,17]],"date-time":"2023-11-17T02:05:09Z","timestamp":1700186709000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ptr.1433"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,4]]},"references-count":18,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2004,4]]}},"alternative-id":["10.1002\/ptr.1433"],"URL":"https:\/\/doi.org\/10.1002\/ptr.1433","archive":["Portico"],"relation":{},"ISSN":["0951-418X","1099-1573"],"issn-type":[{"value":"0951-418X","type":"print"},{"value":"1099-1573","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,4]]}}}