{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T06:38:20Z","timestamp":1773211100880,"version":"3.50.1"},"reference-count":19,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2004,5,18]],"date-time":"2004-05-18T00:00:00Z","timestamp":1084838400000},"content-version":"vor","delay-in-days":47,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Phytotherapy Research"],"published-print":{"date-parts":[[2004,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We have previously reported that tetrandrine reduced hepatic stellate cell activation and collagen accumulation in liver \ufb01brosis induced by biliary obstruction. In the present study, we examined the apoptosis\u2010inducing effect of tetrandrine on activated hepatic stellate cells, as the therapeutic goal in hepatic \ufb01brosis is to eliminate the activated hepatic stellate cells by apoptosis. We used rat hepatic stellate cells transformed by Simian virus 40 (T\u2010HSC\/Cl\u20106) to overcome the limitations inherent in studying primary cultures of hepatic stellate cells. Tetrandrine treatment at doses of 25 and 50 \u00b5g\/ml for 12 h induced apoptosis as con\ufb01rmed by DNA fragmentation and increased sub\u2010G1 DNA content as detected by \ufb02ow cytometric analysis. Tetrandrine also induced the activation of caspase\u20103 protease and subsequent proteolytic cleavage of poly(ADP\u2010ribose) polymerase. In conclusion, our results demonstrate that tetrandrine induces apoptosis of T\u2010HSC\/Cl\u20106 cells, and these results should contribute to the development of new agents for the treatment of hepatic \ufb01brosis. 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