{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,5]],"date-time":"2026-03-05T02:58:04Z","timestamp":1772679484575,"version":"3.50.1"},"reference-count":35,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2005,9,21]],"date-time":"2005-09-21T00:00:00Z","timestamp":1127260800000},"content-version":"vor","delay-in-days":51,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Phytotherapy Research"],"published-print":{"date-parts":[[2005,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Carotenoid fractions were extracted from red paprika, <jats:italic>Valencia orange<\/jats:italic> peel and the peel of <jats:italic>Golden delicious<\/jats:italic> apple. Thus, hypophasic carotenoids of paprika (PM1), orange (PM3) and apple (PM4), and epiphasic extractions of paprika (PM2) and apple (PM5) were obtained by extraction, saponification and partition between MeOH\u2013H<jats:sub>2<\/jats:sub>O (9:1) (hypophasic) and hexane (epiphasic). A high content of capsanthin was quantified in hypophasic carotenoids (PM1) from red spice paprika, whereas the hypophasic fractions from orange (PM3) and apple (PM4) were mainly composed of violaxanthin, zeaxanthin and lutein. On the other hand, a high content of <jats:italic>\u03b2<\/jats:italic>,<jats:italic>\u03b2<\/jats:italic>\u2010carotene and <jats:italic>\u03b2<\/jats:italic>\u2010cryptoxanthin was found in epiphasic fractions (PM2 and PM5). The extracts were studied for their anti\u2010<jats:italic>Helicobacter pylori<\/jats:italic> (<jats:italic>H. pylori<\/jats:italic>), anti\u2010human immunodeficiency virus (HIV), cytotoxic, multidrug resistance (MDR) reversal and radical scavenging activity. Among five PM extracts and <jats:italic>\u03b2<\/jats:italic>,<jats:italic>\u03b2<\/jats:italic>carotene, PM4 showed potent anti\u2010<jats:italic>H. pylori<\/jats:italic> activity (MIC<jats:sub>50<\/jats:sub> = 36 \u00b5g\/mL), comparable to metronidazole (MIC<jats:sub>50<\/jats:sub> = 45 \u00b5g\/mL). The extracts were inactive against HIV. PM3 and PM4 showed slightly higher cytotoxic activity against three human tumor cell lines (squamous cell carcinoma HSC\u20102, HSC\u20103, submandibular gland carcinoma HSG) and human promyelocytic leukemic HL\u201060 cells than against three normal human oral cells (gingival fibroblast HGF, pulp cell HPC, periodontal ligament fibroblast HPLF), suggesting a tumor\u2010specific cytotoxic activity. PM1, PM3 and PM4 displayed much higher MDR\u2010reversing activity than (\u00b1)\u2010verapamil. ESR spectroscopy demonstrated that PM1\u20105 and <jats:italic>\u03b2<\/jats:italic>,<jats:italic>\u03b2<\/jats:italic>\u2010carotene produced little or no detectable radical under alkaline conditions and did not scavenge the O<jats:sub>2<\/jats:sub><jats:sup>\u2212<\/jats:sup> produced by the hypoxanthine and xanthine oxidase reaction. On the other hand, PM1 and PM2 scavenged efficiently 1,1\u2010diphenyl\u20102\u2010picrylhydrazyl (DPPH) radical, whereas singlet oxygen was also quenched efficiently by PM5 and PM2. The data suggest the potential importance of carotenoids as possible anti\u2010<jats:italic>H. pylori<\/jats:italic> and MDR reversal agents. The active principles in the carotenoid extract might differ, depending upon the types of fruits and vegetables. Copyright \u00a9 2005 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/ptr.1735","type":"journal-article","created":{"date-parts":[[2005,9,21]],"date-time":"2005-09-21T14:17:32Z","timestamp":1127312252000},"page":"700-707","source":"Crossref","is-referenced-by-count":32,"title":["Biological activity of carotenoids in red paprika, <i>Valencia orange<\/i> and <i>Golden delicious<\/i> 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