{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T17:15:51Z","timestamp":1789665351282,"version":"4.0.1"},"reference-count":31,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2006,8,14]],"date-time":"2006-08-14T00:00:00Z","timestamp":1155513600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Phytotherapy Research"],"published-print":{"date-parts":[[2006,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The effects of pumpkin seed (<jats:italic>Cucurbita pepo<\/jats:italic>) protein isolate on the plasma activity levels of catalase (CA), superoxide dismutase (SOD), glutathione peroxidase (GSHpx) and total antioxidant capacity (TAC) as well as glucose\u20106\u2010phosphatase (G6Pase) in liver homogenates and lipid peroxidation (LPO\u2010malondialdehyde\u2010MDA) levels in liver homogenates and liver microsomal fractions against carbon tetrachloride (CCl<jats:sub>4<\/jats:sub>)\u2010induced acute liver injury in low\u2010protein fed Sprague\u2010Dawley rats (<jats:italic>Rattus norvegicus<\/jats:italic>) were investigated.<\/jats:p><jats:p>A group of male Sprague\u2010Dawley rats maintained on a low\u2010protein diet for 5 days were divided into three subgroups. Two subgroups were injected with carbon tetrachloride and the other group with an equivalent amount of olive oil. Two hours after CCl<jats:sub>4<\/jats:sub> intoxication one of the two subgroups was administered with pumpkin seed protein isolate and thereafter switched onto a 20% pumpkin seed protein isolate diet. The other two groups of rats were maintained on the low\u2010protein diet for the duration of the investigation. Groups of rats from the different subgroups were killed at 24, 48 and 72 h after their respective treatments. After 5 days on the low\u2010protein diet the activity levels of all the enzymes as well as antioxidant levels were significantly lower than their counterparts on a normal balanced diet. However, a low\u2010protein diet resulted in significantly increased levels of lipid peroxidation. The CCl<jats:sub>4<\/jats:sub> intoxicated rats responded in a similar way, regarding all the variables investigated, to their counterparts on a low\u2010protein diet. The administration of pumpkin seed protein isolate after CCl<jats:sub>4<\/jats:sub> intoxication resulted in significantly increased levels of all the variables investigated, with the exception of the lipid peroxidation levels which were significantly decreased. From the results of the present study it is concluded that pumpkin seed protein isolate administration was effective in alleviating the detrimental effects associated with protein malnutrition and CCl<jats:sub>4<\/jats:sub> intoxication. It is therefore apparent that pumpkin seed protein isolate has components that have antiperoxidative properties. 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