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CCE (4\u201360 \u00b5g\/mL) significantly induced the growth of MCF\u20107 cells, an ER\u2010positive human breast cancer cell line, over that of untreated control cells (<jats:italic>p<\/jats:italic> &lt; 0.05). In the ER competitive binding assay, CCE showed higher affinity with ER<jats:italic>\u03b2<\/jats:italic> compared with ER<jats:italic>\u03b1<\/jats:italic>. To investigate the bioactivities of CCE, which act on bone metabolism, the effects of CCE on the function of osteoblastic MC3T3\u2010E1 cells and the production of local factors in osteoblasts were studied. CCE (4\u201360 \u00b5g\/mL) dose\u2010dependently increased the survival of MC3T3\u2010E1 cells. In addition, CCE (10 and 50 \u00b5g\/mL) increased alkaline phosphatase (ALP) activity, collagen synthesis and osteocalcin secretion in MC3T3\u2010E1 cells. Treatment with CCE (10 and 50 \u00b5g\/mL) prevented apoptosis induced by TNF\u2010<jats:italic>\u03b1<\/jats:italic> (10<jats:sup>\u221210<\/jats:sup> <jats:sc>m<\/jats:sc>) in osteoblastic cells. In the presence of TNF\u2010<jats:italic>\u03b1<\/jats:italic>, culture with CCE (10\u2013100 \u00b5g\/mL) for 48 h inhibited the production of IL\u20106 and nitric oxide in osteoblastic MC3T3\u2010E1 cells. These results suggest that <jats:italic>Cinnamomum cassia<\/jats:italic> has a direct stimulatory effect on bone formation <jats:italic>in vitro<\/jats:italic> and may contribute to the prevention of osteoporosis and inflammatory bone diseases. 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