{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T22:00:44Z","timestamp":1759960844898,"version":"3.33.0"},"reference-count":20,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2007,12,4]],"date-time":"2007-12-04T00:00:00Z","timestamp":1196726400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Phytotherapy Research"],"published-print":{"date-parts":[[2008,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Ranunculus<\/jats:italic>(Crowfoot) species are numerous and they are all reputed to be counter\u2010irritants and are used in several topical conditions. In order to study the pharmacological mechanisms of action underlying this popular use, a methanol extract of<jats:italic>Ranunculus peltatus<\/jats:italic>was tested<jats:italic>in vitro<\/jats:italic>in various assays involving eicosanoid and human elastase release by intact cells as well as<jats:italic>in vivo<\/jats:italic>, with models of delayed\u2010type hypersensitivity (DTH) contact dermatitis. The extract proved to be a selective inhibitor of the cyclooxygenase\u20101 pathway, producing the total inhibition of 12\u2010(<jats:italic>S<\/jats:italic>)\u2010HHTrE release at 200 \u00b5g\/mL, while leaving both 5\u2010lipoxygenase and 12\u2010lipoxygenase activities unaffected at the same dose. The<jats:italic>n<\/jats:italic>\u2010hexane, chloroform and ethyl acetate fractions of the crude methanol extract inhibited LTB<jats:sub>4<\/jats:sub>release by intact rat peritoneal neutrophils, but more polar fractions were inactive and did not increase the 5\u2010LOX activity as seen previously for extracts of other<jats:italic>Ranunculus<\/jats:italic>species. In the<jats:italic>in vivo<\/jats:italic>models, the methanol extract reduced the dinitrofluorobenzene (DNFB)\u2010induced oedema by 40%, but failed to inhibit the oedema brought on by oxazolone. The results agree with the age\u2010old assertion that Water Crowfoot species can be used as a topical antiinflammatory remedy without the prominent irritant action that accompanies the application of non\u2010aquatic<jats:italic>Ranunculus<\/jats:italic>species. Copyright \u00a9 2007 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/ptr.2309","type":"journal-article","created":{"date-parts":[[2007,12,4]],"date-time":"2007-12-04T13:19:07Z","timestamp":1196774347000},"page":"297-302","source":"Crossref","is-referenced-by-count":9,"title":["<i>In vitro<\/i>and<i>in vivo<\/i>effects of<i>Ranunculus peltatus<\/i>subsp.<i>baudotii<\/i>methanol extract on models of eicosanoid production and contact dermatitis"],"prefix":"10.1002","volume":"22","author":[{"given":"J. M.","family":"Prieto","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. C.","family":"Recio","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. M.","family":"Giner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G. 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