{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T19:35:41Z","timestamp":1790537741732,"version":"4.1.0"},"reference-count":23,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2007,12,4]],"date-time":"2007-12-04T00:00:00Z","timestamp":1196726400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Phytotherapy Research"],"published-print":{"date-parts":[[2008,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The present study was undertaken in normotensive anaesthetized male rats that received a continuous perfusion of a chrysin glucoside isolated from the flowers and leaves of <jats:italic>Calycotome villosa<\/jats:italic> subsp <jats:italic>intermedia<\/jats:italic> at a dose of 2.5 mg\/kg, or furosemide (control diuretic) at a dose of 0.5 mg\/kg. Compared with the control rats receiving NaCl (0.9%), the urine flow, glomerular filtration and electrolyte excretion (Na<jats:sup>+<\/jats:sup>, K<jats:sup>+<\/jats:sup>) increased significantly in rats treated with chrysin glucoside (<jats:italic>p<\/jats:italic> &lt; 0.001). A similar effect was observed in the rats perfused with furosemide. Intravenous injections of bolus doses (1\u20133 mg\/kg) of the chrysin glucoside to anaesthetized rats elicited an immediate and dose\u2010dependent decrease in mean arterial blood pressure (MABP). Pretreatment of the rats with the nitric oxide synthase inhibitor, <jats:sc>l<\/jats:sc>\u2010NOArg (10 mg\/kg), reduced partially, but significantly (<jats:italic>p<\/jats:italic> &lt; 0.01), the maximal decrease in MABP elicited by chrysin glucoside. In the rat isolated aorta preparation, chrysin glucoside (10\u2013100 \u00b5<jats:sc>m<\/jats:sc>) inhibited in a concentration\u2010dependent manner the noradrenaline (1 \u00b5<jats:sc>m<\/jats:sc>) induced contractions (IC<jats:sub>50<\/jats:sub> = 52 \u00b5<jats:sc>m<\/jats:sc>). This relaxant activity of chrysin glucoside was significantly reduced by incubation of the endothelium\u2010intact rings with <jats:sc>l<\/jats:sc>\u2010NOArg (100 \u00b5<jats:sc>m<\/jats:sc>), (80 \u00b1 4.7% vs 48 \u00b1 5.06% in the absence of <jats:sc>l<\/jats:sc>\u2010NOArg). In conclusion, these results demonstrate a diuretic and hypotensive action of a chrysin glucoside from <jats:italic>Calycotome villosa<\/jats:italic> in anaesthetized rats and indicating an action on renal function, and an active vascular relaxation mediated partially through nitric oxide release. Copyright \u00a9 2007 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/ptr.2322","type":"journal-article","created":{"date-parts":[[2007,12,4]],"date-time":"2007-12-04T13:19:20Z","timestamp":1196774360000},"page":"356-361","source":"Crossref","is-referenced-by-count":21,"title":["Pharmacological studies on hypotensive, diuretic and vasodilator activities of chrysin glucoside from <i>Calycotome villosa<\/i> in rats"],"prefix":"10.1002","volume":"22","author":[{"given":"K.","family":"Cherkaoui\u2010Tangi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Lachkar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Wibo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N.","family":"Morel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. H.","family":"Gilani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B.","family":"Lyoussi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2007,12,4]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-8741(94)90044-2"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-8741(94)90043-4"},{"key":"e_1_2_1_4_1","volume-title":"Introduction to Flavonoids.","author":"Bohm BA","year":"1998"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(01)35241-2"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1097\/00005344-200002000-00023"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0306-3623(95)02001-2"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0306-3623(93)90159-U"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0704064"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-2999(94)90038-8"},{"key":"e_1_2_1_11_1","first-page":"650","article-title":"Isolation and x\u2010ray crystal structure of tetrahydroisoquinoline alkaloids from Calycotome villosa subsp","volume":"9","author":"El Antri A","year":"2004","journal-title":"intermedia"},{"key":"e_1_2_1_12_1","first-page":"774","article-title":"Isolation and x\u2010ray crystal structure of a new isoquinoline\u2010N\u2010oxide alkaloid from Calycotome villosa subsp","volume":"75","author":"El Antri A","year":"2004","journal-title":"intermedia"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.3390\/90700568"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1081\/CEH-100102671"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/0278-6915(95)00077-1"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1097\/00005344-200501000-00013"},{"key":"e_1_2_1_17_1","first-page":"329","article-title":"Effect of nitro\u2010l\u2010arginine on electrical and mechanical responses to acetylcholine in the superior mesenteric artery from stroke prone hypertensive rat","volume":"23","author":"Ghisdal P","year":"1999","journal-title":"Br J Pharmacol"},{"key":"e_1_2_1_18_1","volume-title":"Med\u2010Checklist 4: A Critical Inventory of Vascular Plants of the Circum\u2010mediterranean Countries; Dicotyledones (Lauraceae Rhamnaceae).","author":"Greuter W","year":"1989"},{"key":"e_1_2_1_19_1","volume-title":"Clinical Chemistry: Principles and Techniques","author":"Henry RJ","year":"1974"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/0306-3623(95)02010-1"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/0140-6736(93)92876-U"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.312.7029.478"},{"key":"e_1_2_1_23_1","first-page":"165","article-title":"Anti\u2010inflammatory, spasmolytic and diuretic effects of a commercially available Solidago gigantean Herb. extract","volume":"45","author":"Leuschener J","year":"1995","journal-title":"Arzneimittelforsch Drugs Res"},{"key":"e_1_2_1_24_1","first-page":"335","article-title":"The flavonoids","volume":"5","author":"Middleton E","year":"1984","journal-title":"Trends Pharmacol Sci"}],"container-title":["Phytotherapy Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fptr.2322","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fptr.2322","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ptr.2322","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,14]],"date-time":"2023-11-14T14:57:26Z","timestamp":1699973846000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ptr.2322"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,12,4]]},"references-count":23,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2008,3]]}},"alternative-id":["10.1002\/ptr.2322"],"URL":"https:\/\/doi.org\/10.1002\/ptr.2322","archive":["Portico"],"relation":{},"ISSN":["0951-418X","1099-1573"],"issn-type":[{"value":"0951-418X","type":"print"},{"value":"1099-1573","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,12,4]]}}}