{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T09:41:38Z","timestamp":1781689298841,"version":"3.54.5"},"reference-count":29,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2008,8,7]],"date-time":"2008-08-07T00:00:00Z","timestamp":1218067200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Phytotherapy Research"],"published-print":{"date-parts":[[2008,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Tilia<\/jats:italic> species are widely used in Europe as medicinal plants. The selective antiproliferative activity of a <jats:italic>Tilia cordata<\/jats:italic> flower dichloromethane extract (DME) on a lymphoma cell line has been reported previously and in order to extend this to other unstudied <jats:italic>Tilia<\/jats:italic> species, the effect of <jats:italic>Tilia<\/jats:italic> \u00d7<jats:italic> viridis<\/jats:italic> DME on the proliferation of tumor and normal murine lymphocytes was investigated. The bioguided fractionation of DME yielded a fraction rich in limonene (L), <jats:italic>\u03b1<\/jats:italic>\u2010pinene and <jats:italic>\u03b2<\/jats:italic>\u2010pinene, which presented a selective antiproliferative action on tumor lymphocytes (EC<jats:sub>50<\/jats:sub> on tumor cells: 3.8 \u00b1 0.2 \u00b5g\/mL; EC<jats:sub>50<\/jats:sub> on normal cells: 205 \u00b1 1.8 \u00b5g\/mL). While all monoterpenes exhibited this activity, limonene proved to be the most active (EC<jats:sub>50<\/jats:sub> on tumor cells: 35 \u00b1 2.0 \u00b5g\/mL; EC<jats:sub>50<\/jats:sub> on normal cells: 72 \u00b1 5.0 \u00b5g\/mL) also exerting a stimulatory effect on non\u2010mitogen stimulated lymphocytes proliferation (% of stimulation respect to control) (mean \u00b1 SEM): L 10 \u00b5g\/mL: 25 \u00b1 1.0%; 20 \u00b5g\/mL: 38.5 \u00b1 2.5%; L 40 \u00b5g\/mL: 41 \u00b1 0.9%; L 60 \u00b5g\/mL: 58.5 \u00b1 3%. <jats:italic>T.<\/jats:italic> \u00d7<jats:italic> viridis<\/jats:italic> may thus constitute a potential source of monoterpenes with immunomodulatory activity. Copyright \u00a9 2008 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/ptr.2524","type":"journal-article","created":{"date-parts":[[2008,8,7]],"date-time":"2008-08-07T14:51:14Z","timestamp":1218120674000},"page":"1520-1526","source":"Crossref","is-referenced-by-count":25,"title":["Effect of <i>Tilia<\/i> \u00d7<i> viridis<\/i> flower extract on the proliferation of a lymphoma cell line and on normal murine lymphocytes: contribution of monoterpenes, especially limonene"],"prefix":"10.1002","volume":"22","author":[{"given":"Maria Gabriela","family":"Manuele","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Graciela","family":"Ferraro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudia","family":"Anesini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2008,8,7]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1056-8719(98)00038-0"},{"key":"e_1_2_1_3_1","first-page":"923","volume-title":"Tratado de Fitomedicina, Bases Cl\u00ednicas y Farmacol\u00f3gicas","author":"Alonso JR","year":"1998"},{"key":"e_1_2_1_4_1","first-page":"329","article-title":"Immunomodulatory activity of Larrea divaricata Cav.","volume":"67","author":"Anesini 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