{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,6]],"date-time":"2025-02-06T04:10:19Z","timestamp":1738815019016,"version":"3.37.0"},"reference-count":12,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2008,12,9]],"date-time":"2008-12-09T00:00:00Z","timestamp":1228780800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Phytotherapy Research"],"published-print":{"date-parts":[[2009,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>This aim of this study was to investigate the crude extract of<jats:italic>Buddleja crispa<\/jats:italic>(Bc.Cr) and its active constituent(s) for their antihypertensive and antispasmodic activities. The Bc.Cr caused a dose\u2010dependent (3\u201310 mg\/kg) fall in mean arterial pressure in rats under anesthesia. In rabbit aorta preparations, Bc.Cr (0.03\u20131 mg\/mL) caused inhibition of high K<jats:sup>+<\/jats:sup>(80 mM) precontractions. The Bc.Cr (0.03\u20131 mg\/mL) also inhibited spontaneous and high K<jats:sup>+<\/jats:sup>precontractions in rabbit jejunum preparations, suggestive of calcium channel blocking (CCB) activity. CCB activity was further confirmed when pretreatment of the tissues with Bc.Cr (0.03\u20130.10 mg\/mL) caused a rightward shift in Ca<jats:sup>++<\/jats:sup>concentration response curves, similar to verapamil. Among the pure compounds, BdI\u2010H3 was more potent against the high K<jats:sup>+<\/jats:sup>than spontaneous contractions and was around eight times more potent than Bc.Cr against the spontaneous contractions while the other two compounds, BdI\u20102 and BH\u20103 were inactive. Activity\u2010directed fractionation revealed that the hexane fraction was more potent against K<jats:sup>+<\/jats:sup>precontractions. These data indicate that Bc.Cr possesses a blood\u2010pressure lowering effect, mediated possibly through CCB, though additional mechanism(s) cannot be ruled out. Among the pure compounds, Bdl\u2010H3 is likely to be the active compound involved in the spasmolytic and possibly BP lowering effect of the parent crude extract. Copyright \u00a9 2008 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/ptr.2659","type":"journal-article","created":{"date-parts":[[2008,12,9]],"date-time":"2008-12-09T23:32:17Z","timestamp":1228865537000},"page":"492-497","source":"Crossref","is-referenced-by-count":6,"title":["Presence of blood\u2010pressure lowering and spasmolytic constituents in<i>Buddleja crispa<\/i>"],"prefix":"10.1002","volume":"23","author":[{"given":"Anwarul H.","family":"Gilani","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ishfaq A.","family":"Bukhari","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rafeeq A.","family":"Khan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abdul J.","family":"Shah","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ijaz","family":"Ahmad","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abdul","family":"Malik","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2008,12,9]]},"reference":[{"key":"e_1_2_1_2_1","first-page":"1","volume-title":"Flora of West Pakistan","author":"Abdullah P","year":"1974"},{"key":"e_1_2_1_3_1","first-page":"68","article-title":"Comparative study of frequency of cough with angiotensin converting enzyme inhibitors and angiotensin receptor blockers in hypertension","volume":"15","author":"Ahmad S","year":"2004","journal-title":"Pak J Cardiol"},{"key":"e_1_2_1_4_1","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1515\/znb-2005-0319","article-title":"Enzyme inhibitory constituents from Buddleja crsipa","volume":"60","author":"Ahmad I","year":"2005","journal-title":"Z Naturforsch"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.1979.59.3.606"},{"volume-title":"Trease and Evans Pharmacognosy","year":"2005","author":"Evans WC","key":"e_1_2_1_6_1"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.pa.17.040177.001053"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0306-3623(91)90331-Y"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1139\/Y07-079"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2004.12.021"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/ptr.2000"},{"key":"e_1_2_1_12_1","doi-asserted-by":"crossref","first-page":"312","DOI":"10.1016\/S0031-6997(25)06867-X","article-title":"Calcium antagonism and calcium entry blockade","volume":"38","author":"Godfraind T","year":"1986","journal-title":"Pharmacol Rev"},{"volume-title":"Guide for the care and use of laboratory animals.","year":"1996","author":"National Research Council","key":"e_1_2_1_13_1"}],"container-title":["Phytotherapy Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fptr.2659","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ptr.2659","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,2,6]],"date-time":"2025-02-06T03:14:17Z","timestamp":1738811657000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ptr.2659"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,12,9]]},"references-count":12,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2009,4]]}},"alternative-id":["10.1002\/ptr.2659"],"URL":"https:\/\/doi.org\/10.1002\/ptr.2659","archive":["Portico"],"relation":{},"ISSN":["0951-418X","1099-1573"],"issn-type":[{"type":"print","value":"0951-418X"},{"type":"electronic","value":"1099-1573"}],"subject":[],"published":{"date-parts":[[2008,12,9]]}}}