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The activation and proliferation of hepatic stellate cells (HSCs) is a key event in liver injury. The fruiting body of <jats:italic>Ganoderma lucidum<\/jats:italic> has long been a popular oriental medicine for treating liver diseases. The aim of this present study was to investigate the antiproliferative effects of the triterpenoid\u2010rich extract (GLT) of <jats:italic>G. lucidum<\/jats:italic> in a cell line of rat HSCs (HSC\u2010T6) stimulated with platelet\u2010derived growth factor (PDGF)\u2010BB. DNA synthesis was investigated by bromodeoxyuridine (BrdU) incorporation. Flow cytometry using propidium iodide (PI) labeling was carried out to analyse the cell cycle distribution and apoptosis. <jats:italic>\u03b1<\/jats:italic>\u2010Smooth muscle actin (<jats:italic>\u03b1<\/jats:italic>\u2010SMA) was used to evaluate extracellular matrix deposition, and western blotting was performed to measure cyclins D1 and D2, and phosphorylation of the PDGF<jats:italic>\u03b2<\/jats:italic>\u2010receptor (PDGF<jats:italic>\u03b2<\/jats:italic>R), Akt and JNK. The results indicated that the GLT attenuated BrdU incorporation in a concentration\u2010dependent manner with an IC<jats:sub>50<\/jats:sub> of 8.52 \u00b1 0.33 \u00b5g\/mL. The inhibitory effect of the GLT was associated with downregulation of cyclins D1 and D2, and PDGF<jats:italic>\u03b2<\/jats:italic>R and Akt phosphorylation, upregulation of JNK phosphorylation, and a reduction in <jats:italic>\u03b1<\/jats:italic>\u2010SMA expression. These results indicated that <jats:italic>G. lucidum<\/jats:italic> inhibits PDGF\u2010BB\u2010activated HSC proliferation possibly through blocking PDGF<jats:italic>\u03b2<\/jats:italic>R phosphorylation, thereby indicating its efficacy for preventing and treating hepatic fibrosis. Copyright \u00a9 2008 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/ptr.2687","type":"journal-article","created":{"date-parts":[[2008,12,23]],"date-time":"2008-12-23T12:22:57Z","timestamp":1230034977000},"page":"833-839","source":"Crossref","is-referenced-by-count":19,"title":["<i>Ganoderma lucidum<\/i> extract attenuates the proliferation of hepatic stellate cells by blocking the PDGF 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