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To our knowledge, only garlic and green tea had available data on the possible inhibition of CYP3A4 metabolism. Metabolic studies were performed with human c\u2010DNA baculovirus expressed CYP3A4. Testosterone was used as a substrate and ketoconazole as a positive quantitative inhibition control. The formation of 6\u2010<jats:italic>\u03b2<\/jats:italic>\u2010OH\u2010testosterone was quantified by a validated HPLC methodology. Green tea was the most potent inhibitor of CYP3A4 metabolism (IC<jats:sub>50<\/jats:sub>: 73 \u00b5g\/mL), followed by<jats:italic>Agaricus,<\/jats:italic>mistletoe and noni juice (1324, 3594, &gt;10 000 \u00b5g\/mL, respectively). All IC<jats:sub>50<\/jats:sub>values were high compared with those determined for crude extracts of other herbal remedies. The IC<jats:sub>50<\/jats:sub>\/IC<jats:sub>25<\/jats:sub>ratios for the inhibiting herbal remedies ranged from 2.15 to 2.67, indicating similar inhibition profiles of the herbal inhibitors of CYP3A4. Garlic and Natto K2 were classified as non\u2010inhibitors. Although<jats:italic>Agaricus<\/jats:italic>, noni juice, mistletoe and green tea inhibited CYP3A4 metabolism<jats:italic>in vitro<\/jats:italic>, clinically relevant systemic or intestinal interactions with CYP3A4 were considered unlikely, except for a probable inhibition of intestinal CYP3A4 by the green tea product. 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