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We applied Cytometric bead array technology for simultaneously measurement of these cytokines in pleurisy induced mice treated with lupeol in graded oral doses. Cytometric bead array uses the sensitivity of amplified fluorescence detection by flowcytometer to measure soluble analytes in a particle based immune assay. This assay can accurately quantitate 5 cytokines in a 50 microlitre sample volume. Oral administration of LUP at doses of 25, 50, 100 and 200 mg\/kg p.o. produced dose related inhibition of IL\u20102, IFN\u2010gamma and TNF\u2010<jats:italic>\u03b1<\/jats:italic> in the pleural exudate with the most significant effect at 100 mg\/kg oral dose. LUP had a non significant inhibitory effect on the levels of IL\u20104 and IL\u20105. 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