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The present study investigated whether TP exerted antihepatofibrotic effects <jats:italic>in vitro<\/jats:italic> and <jats:italic>in vivo<\/jats:italic>. A cell line of rat hepatic stellate cells (HSC\u2010T6) was stimulated with tumor necrosis factor\u2010\u03b1 (TNF\u2010\u03b1) or transforming growth factor (TGF)\u2010\u03b21. The inhibitory effects of TP on the nuclear factor\u2010\u03baB (NF\u03baB) signaling cascade and fibrosis markers, including \u03b1\u2010smooth muscle actin (\u03b1\u2010SMA) and collagen, were assessed. An i<jats:italic>n vivo<\/jats:italic> therapeutic study was conducted in dimethylnitrosamine (DMN)\u2010treated rats. The rats were randomly assigned to one of three groups: control rats, DMN rats receiving vehicle only and DMN rats receiving TP (20 \u03bcg\/kg). Treatment was given by gavage twice daily for 3\u2009weeks starting 1\u2009week after the start of DMN administration. TP (5\u2013100\u2009n<jats:sc>m<\/jats:sc>) concentration\u2010dependently inhibited the NF\u03baB transcriptional activity induced by TNF\u2010\u03b1, lipopolysaccharide and phorbol 12\u2010myristate 13\u2010acetate in HSC\u2010T6 cells. In addition, TP also suppressed TNF\u2010\u03b1 and TGF\u2010\u03b21\u2010induced collagen deposition and \u03b1\u2010SMA secretion in HSC\u2010T6 cells. <jats:italic>In vivo<\/jats:italic>, TP treatment significantly reduced hepatic fibrosis scores, collagen contents, IL\u20106 and TNF\u2010\u03b1 levels, and the number of \u03b1\u2010SMA and NF\u03baB\u2010positive cells in DMN rats. The results showed that TP exerted antifibrotic effects in both HSC\u2010T6 cells and DMN rats. Copyright \u00a9 2011 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/ptr.3381","type":"journal-article","created":{"date-parts":[[2011,12,9]],"date-time":"2011-12-09T09:36:04Z","timestamp":1323423364000},"page":"990-999","source":"Crossref","is-referenced-by-count":20,"title":["Antifibrotic Effects of Triptolide on Hepatic Stellate Cells and Dimethylnitrosamine\u2010intoxicated Rats"],"prefix":"10.1002","volume":"25","author":[{"given":"Lee\u2010Won","family":"Chong","sequence":"first","affiliation":[{"name":"Institute of Clinical Medicine School of Medicine National Yang Ming University  Taipei Taiwan"},{"name":"Division of Hepatology and Gastroenterology Department of Internal Medicine Shin Kong Wu Ho\u2010Su Memorial Hospital  Taipei Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yi\u2010Chao","family":"Hsu","sequence":"additional","affiliation":[{"name":"Institute of Cellular and System Medicine National Health Research Institutes  Miaoli Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yung\u2010Tsung","family":"Chiu","sequence":"additional","affiliation":[{"name":"Department of Medical Research and Education Taichung Veterans General Hospital  Taichung Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kuo\u2010Ching","family":"Yang","sequence":"additional","affiliation":[{"name":"Division of Hepatology and Gastroenterology Department of Internal Medicine Shin Kong Wu Ho\u2010Su Memorial Hospital  Taipei Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yi\u2010Tsau","family":"Huang","sequence":"additional","affiliation":[{"name":"Institute of Traditional Medicine School of Medicine National Yang\u2010Ming University  Taipei Taiwan"},{"name":"National Research Institute of Chinese Medicine  Taipei Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2011,1,7]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.510260316"},{"key":"e_1_2_7_3_1","first-page":"G387","article-title":"Mechanisms of hepatic toxicity. 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