{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T16:07:09Z","timestamp":1772986029910,"version":"3.50.1"},"reference-count":43,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2011,6,30]],"date-time":"2011-06-30T00:00:00Z","timestamp":1309392000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Phytotherapy Research"],"published-print":{"date-parts":[[2012,3]]},"abstract":"<jats:p>The aim of this study was to investigate if armepavine (Arm, C<jats:sub>19<\/jats:sub>H<jats:sub>23<\/jats:sub>O<jats:sub>3<\/jats:sub>N) could exert inhibitory effects against hepatic fibrosis in rats. A cell line of rat hepatic stellate cells (HSC\u2010T6) was stimulated with tumour necrosis factor\u2010\u03b1 (TNF\u2010\u03b1) to evaluate the inhibitory effects of Arm. Rats were injected with thioacetamide (TAA; 300\u2009mg\/kg, intraperitoneally) thrice a week for 4\u2009weeks to induce hepatic fibrosis, with Arm (3 or 10\u2009mg\/kg) given by gavage twice a day. Liver sections were taken for western blotting, fibrosis scoring and immunofluorescence staining. Arm (1\u201310\u2009\u00b5<jats:sc>m<\/jats:sc>) concentration\u2010dependently attenuated TNF\u2010\u03b1\u2010stimulated: (i) protein expressions of \u03b1\u2010smooth muscle actin (\u03b1\u2010SMA), collagen type I and angiopoietin\u20101; (ii) H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> production; and (iii) NF\u2010\u03baB, JunD and C\/EBP\u00df (cytidine\u2010cytidine\u2010adenosine\u2010adenosine\u2010thymidine (CCAAT)\/enhancer binding protein\u2010\u00df (EBP\u00df)) nuclear translocations in HSC\u2010T6 cells. <jats:italic>In vivo<\/jats:italic> Arm treatment significantly reduced plasma aspartate transaminase and alanine transaminase levels, hepatic \u03b1\u2010SMA expression and collagen contents, and fibrosis scores of TAA\u2010injected rats. Moreover, Arm treatment decreased \u03b1\u2010SMA\u2010 and NF\u2010\u03baB\u2010positive cells in immunohistochemical staining, and mRNA expression levels of IL\u20106, TGF\u2010\u00df1, TIMP\u20101, col1\u03b12, iNOS and ICAM\u20101 genes, but up\u2010regulated the metallothionein gene in the livers of TAA\u2010injected rats. Our results indicated that Arm exerted both <jats:italic>in vitro<\/jats:italic> and <jats:italic>in vivo<\/jats:italic> antifibrotic effects in rats, with inhibition of NF\u2010\u03baB, JunD and C\/EBP\u00df pathways. Copyright \u00a9 2011 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/ptr.3539","type":"journal-article","created":{"date-parts":[[2011,6,30]],"date-time":"2011-06-30T15:33:48Z","timestamp":1309448028000},"page":"344-353","source":"Crossref","is-referenced-by-count":25,"title":["Effects of Armepavine Against Hepatic Fibrosis Induced by Thioacetamide in Rats"],"prefix":"10.1002","volume":"26","author":[{"given":"Ting\u2010Chun","family":"Weng","sequence":"first","affiliation":[{"name":"Institute of Traditional Medicine, School of Medicine National Yang\u2010Ming University  Taipei Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chien\u2010Chang","family":"Shen","sequence":"additional","affiliation":[{"name":"Division of Medicinal Chemistry National Research Institute of Chinese Medicine  Taipei Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yung\u2010Tsung","family":"Chiu","sequence":"additional","affiliation":[{"name":"Department 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