{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T14:48:14Z","timestamp":1781707694657,"version":"3.54.5"},"reference-count":30,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2011,12,9]],"date-time":"2011-12-09T00:00:00Z","timestamp":1323388800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Phytotherapy Research"],"published-print":{"date-parts":[[2012,8]]},"abstract":"<jats:p>The present study investigated the radioprotective effect of a multifunctional soy isoflavone, genistein, with the testicular system. Genistein was administered (200\u2009mg\/kg body weight) to male C3H\/HeN mice by subcutaneous injection 24\u2009h prior to pelvic irradiation (5\u2009Gy). Histopathological parameters were evaluated 12\u2009h and 21\u2009days post\u2010irradiation. Genistein protected the germ cells from radiation\u2010induced apoptosis (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05 vs vehicle\u2010treated irradiated mice at 12\u2009h post\u2010irradiation). Genistein significantly attenuated radiation\u2010induced reduction in testis weight, seminiferous tubular diameter, seminiferous epithelial depth and sperm head count in the testes (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05 vs vehicle\u2010treated irradiated mice at 21\u2009days post\u2010irradiation). Repopulation and stem cell survival indices of the seminiferous tubules were increased in the genistein\u2010treated group compared with the vehicle\u2010treated irradiation group at 21\u2009days post\u2010irradiation (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.01). The irradiation\u2010mediated decrease in the sperm count and sperm mobility in the epididymis was counteracted by genistein (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.01), but no effect on the frequency of abnormal sperm was evident. Reactive oxygen species (ROS) were evaluated using DCFDA method and exposure to irradiation elevated ROS levels in the testis and genistein treatment resulted in a significant attenuation of radiation\u2010induced ROS production. The results indicate that genistein protects from testicular dysfunction induced by gamma\u2010irradiation by an antiapoptotic effect and recovery of spermatogenesis. Copyright \u00a9 2011 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/ptr.3689","type":"journal-article","created":{"date-parts":[[2011,12,9]],"date-time":"2011-12-09T12:18:37Z","timestamp":1323433117000},"page":"1119-1125","source":"Crossref","is-referenced-by-count":43,"title":["Genistein Mitigates Radiation\u2010induced Testicular Injury"],"prefix":"10.1002","volume":"26","author":[{"given":"Joong\u2010Sun","family":"Kim","sequence":"first","affiliation":[{"name":"Research Center Dongnam Institute of Radiological and Medical Sciences (DIRAMS)  Busan 619\u2010753 South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyu","family":"Heo","sequence":"additional","affiliation":[{"name":"Research Center Dongnam Institute of Radiological and Medical Sciences (DIRAMS)  Busan 619\u2010753 South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joo\u2010Mi","family":"Yi","sequence":"additional","affiliation":[{"name":"Research Center Dongnam Institute of Radiological and Medical Sciences (DIRAMS)  Busan 619\u2010753 South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eun Ji","family":"Gong","sequence":"additional","affiliation":[{"name":"Research Center Dongnam Institute of Radiological and Medical Sciences (DIRAMS)  Busan 619\u2010753 South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kwangmo","family":"Yang","sequence":"additional","affiliation":[{"name":"Research Center Dongnam Institute of Radiological and Medical Sciences (DIRAMS)  Busan 619\u2010753 South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changjong","family":"Moon","sequence":"additional","affiliation":[{"name":"Department of Veterinary Anatomy, College of Veterinary Medicine and Veterinary Medical Research Center Chonnam National University  Gwangju 500\u2010757 South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sung\u2010Ho","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Veterinary Anatomy, College of Veterinary Medicine and Veterinary Medical Research Center Chonnam National University  Gwangju 500\u2010757 South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2011,12,9]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1006\/abbi.1999.1525"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1053\/clon.2001.9234"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1080\/09553000802317778"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0272.2006.00740.x"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1515\/DMDI.2000.17.1-4.261"},{"key":"e_1_2_6_7_1","doi-asserted-by":"crossref","first-page":"1271","DOI":"10.1158\/1535-7163.1271.3.10","article-title":"Genistein potentiates inhibition of tumor growth by radiation in a prostate cancer orthotopic model","volume":"3","author":"Hillman GG","year":"2004","journal-title":"Mol Cancer Ther"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/jncimonographs\/lgi003"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.3164\/jcbn.40.74"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0272.2009.01023.x"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.taap.2007.06.022"},{"key":"e_1_2_6_12_1","doi-asserted-by":"crossref","first-page":"495","DOI":"10.5694\/j.1326-5377.1999.tb127853.x","article-title":"Reproductive aspects of cancer treatment: an update","volume":"70","author":"Kovacs GT","year":"1999","journal-title":"Med J Aust"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/bio.808"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1248\/bpb.26.308"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/jat.904"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1021\/tx00026a012"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/tox.20328"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-05-1302"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neulet.2008.04.058"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.3109\/10915818909014538"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.85.5.2057"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1093\/ecam\/neh066"},{"key":"e_1_2_6_23_1","first-page":"135","article-title":"Biological properties of genistein. 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