{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,8]],"date-time":"2023-10-08T10:12:38Z","timestamp":1696759958276},"reference-count":30,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2012,3,23]],"date-time":"2012-03-23T00:00:00Z","timestamp":1332460800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Phytotherapy Research"],"published-print":{"date-parts":[[2012,12]]},"abstract":"<jats:p>The medicinal plant <jats:italic>Mucuna pruriens<\/jats:italic>, with reputed anti\u2010snake venom properties has been reported to contain a kunitz\u2010type trypsin inhibitor. This study was undertaken to further evaluate the protease inhibitory potential of gpMuc, a multiform glycoprotein, and other protein fractions from <jats:italic>M. pruriens<\/jats:italic> seeds against trypsin, chymotrypsin, <jats:italic>Echis carinatus<\/jats:italic> snake venom, ecarin and thrombin. The results showed that gpMuc inhibited both trypsin and chymotrypsin activities and was thermally stable, maintaining its trypsin inhibitory activity at temperatures of up to 50\u00b0C. Its structural conformation was also maintained at pH ranges of 4\u20137. Immunoreactivity study confirms that it contains protease\u2010recognizing epitope on one of its isoforms. The whole protein extract of <jats:italic>M. pruriens<\/jats:italic> seeds inhibited prothrombin activation by ecarin and whole <jats:italic>E. carinatus<\/jats:italic> venom, and also thrombin\u2010like activity using chromogenic assay. Copyright \u00a9 2012 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/ptr.4663","type":"journal-article","created":{"date-parts":[[2012,3,24]],"date-time":"2012-03-24T09:07:35Z","timestamp":1332580055000},"page":"1913-1919","source":"Crossref","is-referenced-by-count":2,"title":["Effects of <i>Mucuna pruriens<\/i> Protease Inhibitors on <i>Echis carinatus<\/i> Venom"],"prefix":"10.1002","volume":"26","author":[{"given":"Nnadozie Stanley","family":"Hope\u2010Onyekwere","sequence":"first","affiliation":[{"name":"Department of Internal Medicine, Endocrine\u2010Metabolic Science and Biochemistry University of Siena  Via A. 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