{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,7,22]],"date-time":"2024-07-22T02:30:00Z","timestamp":1721615400556},"reference-count":15,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2012,3,16]],"date-time":"2012-03-16T00:00:00Z","timestamp":1331856000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Korea Healthcare Technology R&D Projects, Minister for Health, Welfare & Family Affairs, Republic of Korea","award":["A090084"],"award-info":[{"award-number":["A090084"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Phytotherapy Research"],"published-print":{"date-parts":[[2013,1]]},"abstract":"<jats:p>The purposes of this study were to determine whether berberine has any effect on phenotype changes and extracellular matrix (ECM) production in nasal polyp\u2010derived fibroblasts (NPDFs) and to investigate the underlying molecular mechanism. NPDFs were pre\u2010treated with berberine prior to induction by transforming growth factor (TGF)\u2010\u03b21. The expression of <jats:italic>\u03b1\u2010smooth muscle actin (SMA)<\/jats:italic> and <jats:italic>collagen type I<\/jats:italic> mRNA was determined by a reverse transcription\u2010polymerase chain reaction, and the expression of \u03b1\u2010SMA protein and collagen type I was determined by western blotting and\/or immunofluorescent staining. The total soluble collagen production was analysed by the SirCol collagen assay. The expression of several signaling molecules of the TGF\u2010\u03b21 pathway was evaluated by western blot analysis. In TGF\u2010\u03b21\u2010induced NPDFs, berberine significantly inhibited the expression of <jats:italic>\u03b1\u2010SMA<\/jats:italic> and <jats:italic>collagen type I<\/jats:italic> mRNA and reduced \u03b1\u2010SMA and collagen protein levels. Berberine only suppressed the expression of pp38 among the evaluated signaling molecules. SB203580 (a specific inhibitor of p38 kinase) markedly suppressed the increased expression of collagen type I and \u03b1\u2010SMA in TGF\u2010\u03b21\u2010induced NPDFs. Berberine exerts suppressive effects on phenotype changes and ECM production in NPDFs via p38 signaling pathway interference. The findings provide new therapeutic options for ECM production in nasal polyps. Copyright \u00a9 2012 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/ptr.4665","type":"journal-article","created":{"date-parts":[[2012,3,16]],"date-time":"2012-03-16T08:04:06Z","timestamp":1331885046000},"page":"16-20","source":"Crossref","is-referenced-by-count":5,"title":["Berberine Inhibits Myofibroblast Differentiation in Nasal Polyp\u2010Derived Fibroblasts via the p38 Pathway"],"prefix":"10.1002","volume":"27","author":[{"given":"You\u2010Mi","family":"Moon","sequence":"first","affiliation":[{"name":"Division of Brain Korea 21 Program for Biomedical Science Korea University College of Medicine  Seoul Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Il\u2010Ho","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology\u2010Head and Neck Surgery Korea University College of Medicine  Seoul 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