{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,29]],"date-time":"2026-03-29T09:25:55Z","timestamp":1774776355630,"version":"3.50.1"},"reference-count":17,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2012,6,2]],"date-time":"2012-06-02T00:00:00Z","timestamp":1338595200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Phytotherapy Research"],"published-print":{"date-parts":[[2013,3]]},"abstract":"<jats:p>Naringenin, a natural predominant flavanone derived from plant food, has antifibrotic activity. The purposes of this study were to determine the effect of naringenin on myofibroblast differentiation and extracellular matrix (ECM) production in nasal polyp\u2010derived fibroblasts (NPDFs) and to determine the molecular mechanism of the effect of naringenin on NPDFs. NPDFs were incubated and treated with transforming growth factor (TGF)\u2010\u03b21. The expression of alpha smooth muscle actin (<jats:italic>\u03b1\u2010SMA), fibronectin<\/jats:italic>, and <jats:italic>collagen type I<\/jats:italic> mRNA was determined by a reverse transcription\u2010polymerase chain reaction, and the expression of those proteins was determined by immunofluorescence staining or Western blotting. Expression of several signaling molecules of the TGF\u2010\u03b21 pathway was evaluated by Western blot analysis. Naringenin inhibits expression of an indicator of myofibroblast differentiation (\u03b1\u2010SMA) and ECM production, including collagen type 1 and fibronectin. Naringenin only suppressed the expression of extracellular signal\u2010regulated protein kinase (pERK)1\/2 among evaluated signaling molecules. PD98059 (a specific inhibitor of ERK1\/2 kinase) also suppressed the increased expression of fibronectin, collagen type I, and \u03b1\u2010SMA in TGF\u2010\u03b21\u2010induced NPDFs. These results suggest the possibility that naringenin may play an inhibitory role in the production of the ECM in the development of nasal polyps. Copyright \u00a9 2012 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/ptr.4735","type":"journal-article","created":{"date-parts":[[2012,6,2]],"date-time":"2012-06-02T06:01:36Z","timestamp":1338616896000},"page":"463-467","source":"Crossref","is-referenced-by-count":9,"title":["Naringenin Inhibits Extracellular Matrix Production via Extracellular Signal\u2010Regulated Kinase Pathways in Nasal Polyp\u2010Derived Fibroblasts"],"prefix":"10.1002","volume":"27","author":[{"given":"Ji Woong","family":"Jung","sequence":"first","affiliation":[{"name":"Department of Otorhinolaryngology\u2010Head and Neck Surgery Korea University Guro Hospital, Korea University College of Medicine  Seoul Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Il\u2010Ho","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology\u2010Head and Neck Surgery Korea University Guro Hospital, Korea University College of Medicine  Seoul Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jung\u2010Sun","family":"Cho","sequence":"additional","affiliation":[{"name":"Division of Brain Korea 21 Program for Biomedical Science Korea University Guro Hospital, Korea University College of Medicine  Seoul Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Heung\u2010Man","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology\u2010Head and Neck Surgery Korea University Guro Hospital, Korea University College of Medicine  Seoul Korea"},{"name":"Division of Brain Korea 21 Program for Biomedical Science Korea University Guro Hospital, Korea University College of Medicine  Seoul Korea"},{"name":"Institute for Medical Devices Clinical Trial Center Korea University Guro Hospital, Korea University College of Medicine  Seoul Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,6,2]]},"reference":[{"key":"e_1_2_7_2_1","first-page":"2084","article-title":"Reconstruction of a human hemicornea through natural scaffolds compatible with the growth of corneal epithelial stem cells and stromal keratocytes","volume":"15","author":"Barbaro V","year":"2009","journal-title":"Mol Vis"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM200005043421807"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/ki.1996.172"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/131.2.235"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-4827(02)00015-0"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1177\/194589240401800307"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1177\/000348940911801007"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M305759200"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1097\/MLG.0b013e318159cc0b"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11095-005-9043-5"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.02554"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1080\/00016489850182945"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1161\/hy0202.103268"},{"key":"e_1_2_7_15_1","first-page":"144","article-title":"Differentiation repertoire of fibroblastic cells: expression of cytoskeletal proteins as marker of phenotypic modulations","volume":"63","author":"Sappino AP","year":"1990","journal-title":"Lab Invest"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.imlet.2006.01.003"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1097\/00005537-199707000-00018"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11010-010-0708-y"}],"container-title":["Phytotherapy Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fptr.4735","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ptr.4735","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,15]],"date-time":"2023-10-15T12:20:55Z","timestamp":1697372455000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ptr.4735"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,6,2]]},"references-count":17,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2013,3]]}},"alternative-id":["10.1002\/ptr.4735"],"URL":"https:\/\/doi.org\/10.1002\/ptr.4735","archive":["Portico"],"relation":{},"ISSN":["0951-418X","1099-1573"],"issn-type":[{"value":"0951-418X","type":"print"},{"value":"1099-1573","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,6,2]]}}}