{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,8,7]],"date-time":"2024-08-07T09:53:03Z","timestamp":1723024383359},"reference-count":36,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2012,7,4]],"date-time":"2012-07-04T00:00:00Z","timestamp":1341360000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Phytotherapy Research"],"published-print":{"date-parts":[[2013,5]]},"abstract":"<jats:p><jats:italic>Desmostachya bipinnata<\/jats:italic>, despite of its popular medicinal uses, has not been widely studied for its effect in diarrhea, indigestion, and asthma. The aim of the present investigation was to provide scientific rationale for these applications. The crude aqueous\u2010methanolic extract of <jats:italic>D. bipinnata<\/jats:italic> (Db.Cr) was evaluated through <jats:italic>in vivo<\/jats:italic> and <jats:italic>in vitro<\/jats:italic> experiments. Db.Cr (100\u2013500 mg\/kg) protected mice against castor oil\u2013induced diarrhea, similar to loperamide. When tested on gut preparations, Db.Cr produced an atropine\u2010sensitive spasmogenic effect in rabbit jejunum up to 5 mg\/mL, followed by a partial relaxation at 10 mg\/mL. With atropine preincubation, a verapamil\u2010like inhibitory effect was evident against spontaneous and high K<jats:sup>+<\/jats:sup> (80 mM)\u2013induced contractions. The maximum stimulant effect was comparable with the acetylcholine\u2010induced maximum contraction and was similarly reproducible in guinea pig ileum. Db.Cr inhibited carbachol (1 \u03bcM)\u2010induced contraction in rabbit trachea but caused an atropine\u2010sensitive accentuation of high K<jats:sup>+<\/jats:sup>\u2013induced contraction at 0.003\u20130.3 mg\/mL followed by inhibition at 1\u20135 mg\/mL. On activity\u2010directed fractionation, inhibitory effect was concentrated on organic and stimulant effect in aqueous fraction. This study, suggesting the presence of calcium antagonist activity, possibly underlying its medicinal effect in hyperactive gut and respiratory disorders, and cholinergic activity, possibly underlying its digestive effect, provides rationale for these therapeutic uses of <jats:italic>D. bipinnata<\/jats:italic>. Copyright \u00a9 2012 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/ptr.4761","type":"journal-article","created":{"date-parts":[[2012,7,5]],"date-time":"2012-07-05T02:31:29Z","timestamp":1341455489000},"page":"678-684","source":"Crossref","is-referenced-by-count":9,"title":["Calcium Channel Blocking Activity in <i>Desmostachya bipinnata<\/i> (L.) Explains its use in Gut and Airways Disorders"],"prefix":"10.1002","volume":"27","author":[{"given":"Hafiz Muhammad","family":"Abdur Rahman","sequence":"first","affiliation":[{"name":"Department of Pharmacy Bahauddin Zakariya University  Multan Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Samra","family":"Bashir","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Bahauddin Zakariya University  Multan Pakistan"},{"name":"Natural Product Research Unit, Department of Biological and Biomedical Sciences, Medical College Aga Khan University  Karachi 74800 Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anwarul Hassan","family":"Gilani","sequence":"additional","affiliation":[{"name":"Natural Product Research Unit, Department of Biological and Biomedical Sciences, Medical College Aga Khan University  Karachi 74800 Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,7,4]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/ptr.3634"},{"key":"e_1_2_7_3_1","first-page":"73","article-title":"Common Weeds of Wheat and Their Control","volume":"7","author":"Ahmad R","year":"2003","journal-title":"Pak J Water Resources"},{"key":"e_1_2_7_4_1","first-page":"76","article-title":"Anti\u2010ulcerogenic Activity of Extract and Some Isolated Flavonoids from Desmostachya bipinnata (L.) 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