{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,3,4]],"date-time":"2024-03-04T18:40:43Z","timestamp":1709577643754},"reference-count":27,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2012,7,17]],"date-time":"2012-07-17T00:00:00Z","timestamp":1342483200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Phytotherapy Research"],"published-print":{"date-parts":[[2013,5]]},"abstract":"<jats:p>Disaccharide intolerance is the inability to digest certain carbohydrates due to a lack of one or more intestinal disaccharidases (e.g., lactase, maltase, isomaltase and sucrase). Symptoms include diarrhea, abdominal distention and flatulence. Management of the disorder by external enzymes supplementation has not yet been attempted. We report that the medicinal plant <jats:italic>Tinospora cordifolia<\/jats:italic> contains substantial amounts of all disaccharidases required for intestinal digestion of carbohydrates. The plant is also a rich source of saccharifying amylase. We recovered (units\/100 g fresh stem) amylase: 49,000\u2009<jats:styled-content>+<\/jats:styled-content>\u2009500, maltase: 400\u2009<jats:styled-content>+<\/jats:styled-content>\u200950, isomaltase: 130\u2009<jats:styled-content>+<\/jats:styled-content>\u200950, sucrase: 4500\u2009<jats:styled-content>+<\/jats:styled-content>\u2009500, acid lactase: 350\u2009<jats:styled-content>+<\/jats:styled-content>\u200930, cellobiase: 35\u2009<jats:styled-content>+<\/jats:styled-content>\u200910 and trehalase: 40\u2009<jats:styled-content>+<\/jats:styled-content>\u200910 by buffer extraction of the blended stem. Crude enzymes in the forms of stem powder, lyophilized aqueous extract and ethanol precipitated protein were found to be stable. Disaccharidases were optimally active at 50 <jats:sup>0<\/jats:sup>C in the pH range of 4\u20135. Lactase was an acid lactase similar to the type linked with human lactose intolerance. Enzymes were catalytically stable in the pH range of 2\u20137 and temperature range of up to 40 <jats:sup>0<\/jats:sup>C. <jats:italic>T. cordifolia<\/jats:italic> enzyme was non\u2010toxic up to a dose of 200 mg protein\/kg body weight. 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