{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,9]],"date-time":"2025-11-09T03:17:21Z","timestamp":1762658241959,"version":"3.40.3"},"reference-count":49,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2012,8,30]],"date-time":"2012-08-30T00:00:00Z","timestamp":1346284800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Phytotherapy Research"],"published-print":{"date-parts":[[2013,6]]},"abstract":"<jats:p><jats:italic>Cissampelos sympodialis<\/jats:italic>Eichl. (Menispermaceae) root infusion is used in Northeast Brazil to treat allergic asthma. We have previously shown that oral use of the plant extract reduces eosinophil infiltration into the lung of ovalbumin (OVA)\u2010 sensitized mice. However, drugs taken by inhalation route to treat asthma achieve better outcomes. Thereby, in this study, we evaluated the inhaled<jats:italic>C. sympodialis<\/jats:italic>alcoholic extract as a therapeutic treatment in OVA\u2010sensitized BALB\/c mice. The parameters which were analyzed consisted of leukocyte recruitment to the airway cavity, tissue remodeling and cell profile. The inhaled extract inhibited mainly eosinophil recruitment to the pleural cavity, bronchoalveolar lavage and peripheral blood. This treatment reduced the OVA\u2010specific IgE serum titer and leukocyte infiltration in the peribronchiolar and pulmonary perivascular areas as well as mucus production. In addition, we also tested isolated alkaloids from the plant extract. The flow cytometric analysis showed that methylwarifteine (MW) and, mainly, the inhaled extract reduced the number of CD3+T cells and eosinophil\u2010like cells. Therefore, inhaled<jats:italic>C. sympodialis<\/jats:italic>extract and MW lead to down\u2010regulation of inflammatory cell infiltration with remarkable decrease in the number of T cells in an experimental model of respiratory allergy, suggesting that the plant can be delivered via inhalation route to treat allergic asthma. Copyright \u00a9 2012 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/ptr.4791","type":"journal-article","created":{"date-parts":[[2012,8,30]],"date-time":"2012-08-30T08:27:10Z","timestamp":1346315230000},"page":"916-925","source":"Crossref","is-referenced-by-count":21,"title":["Inhaled<i>Cissampelos sympodialis<\/i>Down\u2010Regulates Airway Allergic Reaction by Reducing Lung CD3<sup>+<\/sup>T Cells"],"prefix":"10.1002","volume":"27","author":[{"given":"Giciane C.","family":"Vieira","sequence":"first","affiliation":[{"name":"Laboratory of Immunopharmacology, Department of Physiology and Pathology Federal University of Para\u00edba Jo\u00e3o Pessoa Para\u00edba Brazil 58051\u2010970"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Josenilson F.","family":"De Lima","sequence":"additional","affiliation":[{"name":"Laboratory of Immunopharmacology, Department of Physiology and Pathology Federal University of Para\u00edba Jo\u00e3o Pessoa Para\u00edba Brazil 58051\u2010970"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Regina C. 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