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The purpose of this study was twofold: (i) to determine the effect of EGCG on fibroblast differentiation into myofibroblasts and extracellular matrix accumulation in transforming growth factor (TGF)\u2010\u03b21\u2010induced nasal polyp\u2010derived fibroblasts (NPDFs) and (ii) to determine if the antioxidative effect of EGCG on reactive oxygen species (ROS) production in TGF\u2010\u03b21\u2010induced NPDFs is involved in the aforementioned processes. TGF\u2010\u03b21\u2010induced NPDFs were treated with or without EGCG. \u03b1\u2010smooth muscle actin (\u03b1\u2010SMA) and collagen type I mRNA were analyzed by reverse transcription\u2010polymerase chain reaction. \u03b1\u2010SMA protein was also detected using immunofluorescent staining. The amount of total soluble collagen was analyzed by Sircol collagen assay. ROS activity was measured by the nitroblue tetrazolium reduction assay and visualized by fluorescent microscopy. EGCG significantly inhibited expressions of \u03b1\u2010SMA and collagen type I mRNA and reduced \u03b1\u2010SMA and collagen protein levels at concentrations of 10\u201320 \u00b5g\/mL. EGCG also inhibited TGF\u2010\u03b21\u2010induced ROS production at the same concentrations. These results suggest the possibility that EGCG may be effective at inhibiting the development of nasal polyps through an anti\u2010oxidant effect. 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