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In the present study, we found that ethyl<jats:italic>p<\/jats:italic>\u2010methoxycinnamate significantly decreased melanin synthesis in B16F10 murine melanoma cells stimulated with \u03b1\u2010melanocyte stimulating hormone (\u03b1\u2010MSH). In a cell\u2010free system, however, ethyl<jats:italic>p<\/jats:italic>\u2010methoxycinnamate did not directly inhibit tyrosinase, the rate\u2010limiting enzyme of melanogenesis. Instead, it inhibited tyrosinase activity in B16F10 cells in a dose\u2010dependent manner. Furthermore, Western blot analysis showed that ethyl<jats:italic>p<\/jats:italic>\u2010methoxycinnamate decreased microphthalmia\u2010associated transcription factor and tyrosinase levels in \u03b1\u2010MSH\u2010stimulated B16F10 cells. These results indicate that the pigment\u2010inhibitory effect of ethyl<jats:italic>p<\/jats:italic>\u2010methoxycinnamate results from downregulation of tyrosinase. Ethyl<jats:italic>p<\/jats:italic>\u2010methoxycinnamate isolated from<jats:italic>K. galanga<\/jats:italic>could be developed as a skin whitening agent to treat hyperpigmentary disorders. 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