{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T19:52:02Z","timestamp":1792525922184,"version":"4.5.0"},"reference-count":26,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2013,11,8]],"date-time":"2013-11-08T00:00:00Z","timestamp":1383868800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Phytotherapy Research"],"published-print":{"date-parts":[[2014,6]]},"abstract":"<jats:p>Cutaneous leishmaniasis (CL) manifests as localized skin lesions, which lead to significant tissue destruction and disfigurement. In the Yucatan Peninsula, Mayan traditional healers use <jats:italic>Pentalinon andrieuxii<\/jats:italic> Muell.\u2010Arg. (Apocynaceae) roots for the topical treatment of CL. Here, we studied the effect of <jats:italic>P.\u2009andrieuxii<\/jats:italic> root hexane extract (PARE) on the parasites and host cells <jats:italic>in vitro<\/jats:italic> and examined its efficacy in the topical treatment of CL caused by <jats:italic>Leishmania mexicana<\/jats:italic>. PARE exhibited potent antiparasitic activity <jats:italic>in vitro<\/jats:italic> against promastigotes as well as amastigotes residing in macrophages. Electron microscopy of PARE\u2010treated parasites revealed direct membrane damage. PARE also activated nuclear factor kappaB and enhanced interferon\u2010\u03b3 receptor and MHC class II expression and TNF\u2010\u03b1 production in macrophages. In addition, PARE induced production of the Th1 promoting cytokine IL\u201012 in dendritic cells as well as enhanced expression of the co\u2010stimulatory molecules CD40, CD80, and CD86. <jats:italic>In vivo<\/jats:italic> studies showed that <jats:italic>L<\/jats:italic>.\u2009<jats:italic>mexicana<\/jats:italic>\u2010infected mice treated by topical application of PARE resulted in the significant reduction in lesion size and parasite burden compared to controls. These findings indicate that PARE could be used as an alternative therapy for the topical treatment of CL. Copyright \u00a9 2013 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/ptr.5079","type":"journal-article","created":{"date-parts":[[2013,11,8]],"date-time":"2013-11-08T09:27:05Z","timestamp":1383902825000},"page":"909-916","source":"Crossref","is-referenced-by-count":26,"title":["<i>Pentalinon andrieuxii<\/i> Root Extract is Effective in the Topical Treatment of Cutaneous Leishmaniasis Caused by <i>Leishmania mexicana<\/i>"],"prefix":"10.1002","volume":"28","author":[{"given":"Claudio M.","family":"Lezama\u2010D\u00e1vila","sequence":"first","affiliation":[{"name":"Department of Pathology, Wexner Medical Center The Ohio State University  Columbus OH 43210 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Pan","sequence":"additional","affiliation":[{"name":"Division of Medicinal Chemistry and Pharmacognosy, College of Pharmacy The Ohio State University  Columbus OH 43210 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angelica P.","family":"Isaac\u2010M\u00e1rquez","sequence":"additional","affiliation":[{"name":"Centro De Investigaci\u00f3nes Biomedicas Universidad Aut\u00f3noma de Campeche  Campeche 24030 Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cesar","family":"Terrazas","sequence":"additional","affiliation":[{"name":"Department of Pathology, Wexner Medical Center The Ohio State University  Columbus OH 43210 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steve","family":"Oghumu","sequence":"additional","affiliation":[{"name":"Department of Pathology, Wexner Medical Center The Ohio State University  Columbus OH 43210 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ricardo","family":"Isaac\u2010M\u00e1rquez","sequence":"additional","affiliation":[{"name":"Centro de Desarrollo Sustentable Universidad Aut\u00f3noma de Campeche  Campeche 24030 Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"MY","family":"Pech\u2010Dzib","sequence":"additional","affiliation":[{"name":"Centro De Investigaci\u00f3nes Biomedicas Universidad Aut\u00f3noma de Campeche  Campeche 24030 Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph","family":"Barbi","sequence":"additional","affiliation":[{"name":"Department of Oncology, Immunology and Hematopoiesis Division, Sidney Kimmel Comprehensive Cancer Center Johns Hopkins University School of Medicine  Baltimore MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edward","family":"Calomeni","sequence":"additional","affiliation":[{"name":"Department of Pathology, Wexner Medical Center The Ohio State University  Columbus OH 43210 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Narasimham","family":"Parinandi","sequence":"additional","affiliation":[{"name":"Pulm, Allergy, Critical Care &amp; Sleep, College of Medicine The Ohio State University  Columbus OH 43210 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. 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