{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,16]],"date-time":"2025-12-16T12:21:03Z","timestamp":1765887663732},"reference-count":34,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2015,1,14]],"date-time":"2015-01-14T00:00:00Z","timestamp":1421193600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Phytotherapy Research"],"published-print":{"date-parts":[[2015,4]]},"abstract":"<jats:p><jats:italic>Acanthopanax senticosus<\/jats:italic> (Rupr. et Maxim.) Harms (AS), a traditional herbal medicine, has been widely used to treat ischemic heart disease. However, the underlying cellular mechanisms of its benefits to cardiac function remain unclear. The present study examined the effects of total flavones from AS (TFAS) on L\u2010type Ca<jats:sup>2+<\/jats:sup> channel currents (I<jats:sub>Ca\u2010L<\/jats:sub>) using the whole cell patch\u2010clamp technique and on intracellular calcium ([Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>) handling and cell contractility in rat ventricular myocytes with the aid of a video\u2010based edge\u2010detection system. Exposure to TFAS resulted in a concentration\u2010 and voltage\u2010dependent blockade of I<jats:sub>Ca\u2010L<\/jats:sub>, with the half\u2010maximal inhibitory concentration (IC<jats:sub>50<\/jats:sub>) of 283.12\u2009\u00b5g\/mL and the maximal inhibitory effect of 36.49\u2009\u00b1\u20091.95%. Moreover, TFAS not only increased the maximum current in the current\u2013voltage relationship but also shifted the activation and inactivation curves of I<jats:sub>Ca\u2010L<\/jats:sub> toward the hyperpolarizing direction. Meanwhile, TFAS significantly reduced amplitudes of myocyte shortening and [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> with an increase in the time to 10% of the peak (Tp) and a decrease in the time to 10% of the baseline (Tr). Thus, the cardioprotective effects of TFAS may be attributed mainly to the attenuation of [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> through the direct inhibition of I<jats:sub>Ca\u2010L<\/jats:sub> in rat ventricular myocytes and consequent negative effect on myocardial contractility. Copyright \u00a9 2015 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/ptr.5278","type":"journal-article","created":{"date-parts":[[2015,1,14]],"date-time":"2015-01-14T06:52:56Z","timestamp":1421218376000},"page":"533-539","source":"Crossref","is-referenced-by-count":22,"title":["Effects of Total Flavones from <i>Acanthopanax senticosus<\/i> on L\u2010type Calcium Channels, Calcium Transient and Contractility in Rat Ventricular Myocytes"],"prefix":"10.1002","volume":"29","author":[{"given":"Shengjiang","family":"Guan","sequence":"first","affiliation":[{"name":"Department of Pharmacology Hebei University of Chinese Medicine  Shijiazhuang 050200 Hebei China"},{"name":"Department of Pharmacy The Hospital of Hebei University of Chinese Medicine  Shijiazhuang 050011 Hebei China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juanjuan","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Pharmacology Hebei University of Chinese Medicine  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