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In the present study, five bioactive characteristics of olive oil by\u2010products were assessed, namely their antioxidant, anti\u2010bacterial, anti\u2010melanogenesis, anti\u2010allergic, and collagen\u2010production\u2010promoting activities. First, the extracts of leaves (May and October), stems (May and October), flowers, olive milled waste, fruit pulp and seeds were prepared using two safe solvents, ethanol and water. According to HPLC and LC\/MS analysis and Folin\u2013Ciocalteu assay, the ethanol extracts of the leaves (May and October), stems (May and October) and flowers contained oleuropein, and the ethanol extract of the stems showed the highest total phenol content. Oleuropein may contribute to the antioxidant and anti\u2010melanogenesis activities of the leaves, stems, and flowers. However, other active compounds or synergistic effects present in the ethanol extracts are also likely to contribute to the anti\u2010bacterial activity of the leaves and flowers, the anti\u2010melanogenesis activity of some parts, the anti\u2010allergic activity of olive milled waste, and the collagen\u2010production\u2010promoting activity of the leaves, stems, olive milled waste and fruit pulp. This study provides evidence that the by\u2010products of olive oil have the potential to be further developed and used in the skin care industry. Copyright \u00a9 2015 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/ptr.5326","type":"journal-article","created":{"date-parts":[[2015,3,16]],"date-time":"2015-03-16T06:28:43Z","timestamp":1426487323000},"page":"877-886","source":"Crossref","is-referenced-by-count":40,"title":["Multiple Biological Effects of Olive Oil By\u2010products such as Leaves, Stems, Flowers, Olive Milled Waste, Fruit Pulp, and Seeds of the Olive Plant on Skin"],"prefix":"10.1002","volume":"29","author":[{"given":"Asuka","family":"Kishikawa","sequence":"first","affiliation":[{"name":"Department of Agro\u2010environmental Sciences, Faculty of Agriculture Kyushu University  Fukuoka 812\u20108581 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmed","family":"Ashour","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy, Faculty of Pharmacy Mansoura University  Mansoura Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qinchang","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Agro\u2010environmental Sciences, Faculty of Agriculture Kyushu University  Fukuoka 812\u20108581 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Midori","family":"Yasuda","sequence":"additional","affiliation":[{"name":"Department of Health and Nutrition Science Nishikyushu University  Saga 842\u20108585 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroya","family":"Ishikawa","sequence":"additional","affiliation":[{"name":"International College of Arts and Sciences Fukuoka Women's University  Fukuoka 813\u20108529 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kuniyoshi","family":"Shimizu","sequence":"additional","affiliation":[{"name":"Department of Agro\u2010environmental Sciences, Faculty of Agriculture Kyushu University  Fukuoka 812\u20108581 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,3,16]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/ptr.1363"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbdv.200790173"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2010.07.067"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.indcrop.2013.04.042"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/21.11.2085"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ab.2008.09.052"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.arr.2013.12.003"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms13033291"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1021\/np400175b"},{"key":"e_1_2_7_11_1","first-page":"1193","article-title":"Photo\u2010protective and anti\u2010melanogenic effect from phenolic compound of olive leaf (Olea europaea L. var. 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