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The prokinetic, laxative, and antidiarrheal activities of<jats:italic>M.\u2009nigra<\/jats:italic>were assessed in mice, while isolated rabbit jejunum and guinea\u2010pig ileum were used to explore insight into mechanism(s). At 30 and 70\u2009mg\/kg, the crude extract of<jats:italic>M.\u2009nigra<\/jats:italic>(Mn.Cr) exhibited atropine\u2010sensitive prokinetic and laxative effects, similar to carbachol (CCh). While at higher doses (100, 300, and 500\u2009mg\/kg), Mn.Cr offered protection against castor oil\u2010induced diarrhea. In rabbit jejunum, Mn.Cr and its chloroform fraction inhibited CCh\u2010induced contractions more potently compared with high K<jats:sup>+<\/jats:sup>(80\u2009m<jats:sc>m<\/jats:sc>). Conversely, petroleum fraction was more potent against high\u2010K<jats:sup>+<\/jats:sup>\u2010induced contractions. At 0.01\u2009mg\/mL, Mn.Cr caused a parallel shift in acetylcholine concentration\u2013response curves (CRCs) followed by a non\u2010parallel shift at 0.03\u2009mg\/mL, similar to dicyclomine. At further tested concentrations, Mn.Cr (0.1 and 0.3\u2009mg\/mL) and petroleum fraction suppressed Ca<jats:sup>2+<\/jats:sup>CRCs, similar to verapamil. In guinea\u2010pig ileum, Mn.Cr, its aqueous and ethyl acetate fractions exhibited atropine\u2010sensitive gut stimulant activity along with additional uncharacterized excitatory response in the aqueous fraction only. These results suggest that black mulberry possesses prokinetic, laxative, and antidiarrheal effects, putatively mediated through cholinomimetic, antimuscarinic, and Ca<jats:sup>2+<\/jats:sup>antagonist mechanisms, respectively. 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