{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T22:50:56Z","timestamp":1783464656042,"version":"3.55.0"},"reference-count":20,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2017,6,22]],"date-time":"2017-06-22T00:00:00Z","timestamp":1498089600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100011080","name":"Department of Plant Sciences, University of California, Davis","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100011080","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Phytotherapy Research"],"published-print":{"date-parts":[[2017,9]]},"abstract":"<jats:p>The cape myrtle, <jats:italic>Myrsine africana<\/jats:italic> L., is a widely used medicinal plant, which has not been well investigated. We assessed the <jats:italic>in vivo<\/jats:italic> hepatoprotective and <jats:italic>in vitro<\/jats:italic> antiproliferative and antioxidant effects of leaf extracts of <jats:italic>M. africana<\/jats:italic> chemically profiled using high\u2010performance liquid chromatography. Three flavonoids were quantified, and gas chromatography\u2013mass spectrometry analysis revealed the presence of common fatty acids. The animal study was conducted on mice treated with CCl4, using three doses each of the methanol and chloroform extract (100, 200 and 300 mg\/kg b.w.),with silymarin as a positive control. Hepatoprotective effects were determined by analyzing blood for liver marker and antioxidant enzymes, direct bilirubins and total proteins. The methanol extract (300 mg\/kg b.w.) showed the strongest hepatoprotective effects against abnormalities produced by CCl4. The <jats:italic>in vivo<\/jats:italic> hepatoprotective effects correlated well with the <jats:italic>in vitro<\/jats:italic> antioxidant and antiproliferative activities and with high levels of flavonoids in the extracts. Finally, molecular docking studies of the constituent quercetin were undertaken <jats:italic>in silico<\/jats:italic> and several sites of binding to human estrogen receptor (ER) protein, linked with alkaline phosphatase, identified. Copyright \u00a9 2017 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/ptr.5853","type":"journal-article","created":{"date-parts":[[2017,6,22]],"date-time":"2017-06-22T08:33:19Z","timestamp":1498120399000},"page":"1305-1309","source":"Crossref","is-referenced-by-count":10,"title":["The <i>In Vitro<\/i> and <i>In Vivo<\/i> Biological Activities of the Leaf of Cape Myrtle, <i>Myrsine africana<\/i> L."],"prefix":"10.1002","volume":"31","author":[{"given":"Hina","family":"Gul","sequence":"first","affiliation":[{"name":"Department of Plant Sciences Faculty of Biological Sciences Quaid\u2010i\u2010Azam University  Islamabad Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mushtaq","family":"Ahmad","sequence":"additional","affiliation":[{"name":"Department of Plant Sciences Faculty of Biological Sciences Quaid\u2010i\u2010Azam University  Islamabad Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad","family":"Zafar","sequence":"additional","affiliation":[{"name":"Department of Plant Sciences Faculty of Biological Sciences Quaid\u2010i\u2010Azam University  Islamabad Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad","family":"Sheeraz Ahmad","sequence":"additional","affiliation":[{"name":"Department of Biochemistry PMAS Arid Agriculture  Rawalpindi Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ambreen","family":"Abid","sequence":"additional","affiliation":[{"name":"Department of Biochemistry PMAS Arid Agriculture  Rawalpindi Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Syeda","family":"Hira","sequence":"additional","affiliation":[{"name":"Department of Biochemistry PMAS Arid Agriculture  Rawalpindi Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Imam","family":"Shah","sequence":"additional","affiliation":[{"name":"National Veterinary Laboratory  Islamabad Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4269-6187","authenticated-orcid":false,"given":"Muhammad","family":"Gulfraz","sequence":"additional","affiliation":[{"name":"Department of Chemistry COMSATS  Abbottabad Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,6,22]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2014.12.051"},{"key":"e_1_2_7_3_1","first-page":"1","article-title":"In vitro and in vivo anticancer activity of root extract of Sansevieria liberica Gerome and Labrory (Agavaccae)","volume":"2015","author":"Abidemi JA","year":"2015","journal-title":"Evid Based Complement Alternat Med"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.2174\/1876142911002020166"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(84)05016-3"},{"issue":"6","key":"e_1_2_7_6_1","first-page":"1977","article-title":"Antibacterial activity of crude methanolic extract and various fractions of Vitex agnuscastus and Myrsine africana against clinical isolates of methicillin resistant \n                     Staphylococcus aureus","volume":"29","author":"Ahmad B","year":"2016","journal-title":"Pak J Pharm Sci"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fct.2010.04.029"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1186\/1472-6882-11-55"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(71)90370-8"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf052632g"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2003.11.009"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2011.08.074"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(84)05015-1"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/ptr.2438"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcc.21498"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)52451-6"},{"key":"e_1_2_7_17_1","doi-asserted-by":"crossref","first-page":"3410","DOI":"10.1016\/S0021-9258(19)45155-7","article-title":"The purification and properties of superoxide dismutase from Neurosporacrassa","volume":"247","author":"Misra HP","year":"1972","journal-title":"J Biol Chem"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf803537k"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcc.21256"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1021\/np200906s"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioorg.2016.10.002"}],"container-title":["Phytotherapy Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fptr.5853","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ptr.5853","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,5]],"date-time":"2023-10-05T02:47:58Z","timestamp":1696474078000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ptr.5853"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,6,22]]},"references-count":20,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2017,9]]}},"alternative-id":["10.1002\/ptr.5853"],"URL":"https:\/\/doi.org\/10.1002\/ptr.5853","archive":["Portico"],"relation":{},"ISSN":["0951-418X","1099-1573"],"issn-type":[{"value":"0951-418X","type":"print"},{"value":"1099-1573","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,6,22]]}}}