{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,4]],"date-time":"2025-10-04T07:53:40Z","timestamp":1759564420839,"version":"3.41.0"},"reference-count":52,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2017,8,25]],"date-time":"2017-08-25T00:00:00Z","timestamp":1503619200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Phytotherapy Research"],"published-print":{"date-parts":[[2017,11]]},"abstract":"<jats:p><jats:styled-content style=\"fixed-case\"><jats:italic>Achyranthes aspera<\/jats:italic><\/jats:styled-content>L. is traditionally used to relieve constipation, diarrhea, and asthma. Its crude extract (Aa.Cr) was evaluated through<jats:italic>in vivo<\/jats:italic>and<jats:italic>ex vivo<\/jats:italic>experiments to rationalize these medicinal uses of<jats:styled-content style=\"fixed-case\"><jats:italic>A. aspera<\/jats:italic><\/jats:styled-content>and to provide their scientific basis. Aa.Cr, at 3 and 10\u00a0mg\/kg, increased fecal output, similar to castor oil, whereas at 30, 100, 300, and 700\u00a0mg\/kg, it protected against castor oil\u2010induced diarrhea in mice when administered orally. Aa.Cr caused spasmogenic effect on rabbit jejunum and guinea pig ileum preparations, which was partially inhibited by atropine while completely blocked by cyproheptadine preincubation. Aa.Cr also relaxed high K<jats:sup>+<\/jats:sup>(80\u00a0mM)\u2010induced contraction in rabbit jejunum. Aa.Cr inhibited CCh (100\u00a0\u03bcg\/kg)\u2010induced bronchospasm in rats, similar to aminophylline. Like dicyclomine, Aa.Cr relaxed high K<jats:sup>+<\/jats:sup>and CCh (1\u00a0\u03bcM)\u2010induced contractions in guinea pig trachea and caused rightwards parallel shift of CCh concentration\u2013response curves at the lower concentrations followed by non\u2010parallel shift at the higher concentrations. On activity\u2010directed fractionation, spasmogenic and spasmolytic activities of Aa.Cr were concentrated in aqueous and organic fraction, respectively. This study suggests the presence of dose\u2010specific laxative and antidiarrheal effects in<jats:italic>A. aspera<\/jats:italic>, possibly mediated through cyproheptadine\u2010sensitive receptors and dual cholinergic and calcium channel blockade, respectively. The latter combination is also a suggested mechanism underlying its bronchodilator effect. Copyright \u00a9 2017 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/ptr.5907","type":"journal-article","created":{"date-parts":[[2017,8,25]],"date-time":"2017-08-25T08:22:46Z","timestamp":1503649366000},"page":"1776-1785","source":"Crossref","is-referenced-by-count":8,"title":["Pharmacological Evaluation of Gut Modulatory and Bronchodilator Activities of<scp><i>Achyranthes aspera<\/i><\/scp>Linn."],"prefix":"10.1002","volume":"31","author":[{"given":"Hafiz Muhammad Abdur","family":"Rahman","sequence":"first","affiliation":[{"name":"Natural Product Research Division, Department of Biological and Biomedical Sciences Aga Khan University Medical College Karachi 74800 Pakistan"},{"name":"Faculty of Pharmacy Bahauddin Zakariya University Multan Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6972-8284","authenticated-orcid":false,"given":"Samra","family":"Bashir","sequence":"additional","affiliation":[{"name":"Natural Product Research Division, Department of Biological and Biomedical Sciences Aga Khan University Medical College Karachi 74800 Pakistan"},{"name":"M. 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