{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T03:35:21Z","timestamp":1778556921371,"version":"3.51.4"},"reference-count":30,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2017,10,11]],"date-time":"2017-10-11T00:00:00Z","timestamp":1507680000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Southwest University: the Fundamental Research Funds for the Central Universities","award":["XDJK2017D115 and XDJK2016E104"],"award-info":[{"award-number":["XDJK2017D115 and XDJK2016E104"]}]},{"name":"Chongqing Education Commission: the Graduate Research and Innovation Project of Chongqing","award":["CYS16077 and CYS2015069"],"award-info":[{"award-number":["CYS16077 and CYS2015069"]}]},{"name":"Department of Agriculture: the National Sericulture Industry Technology System","award":["CARS-22-ZJ0204"],"award-info":[{"award-number":["CARS-22-ZJ0204"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Phytotherapy Research"],"published-print":{"date-parts":[[2017,12]]},"abstract":"<jats:p><jats:styled-content style=\"fixed-case\"><jats:italic>Morus alba<\/jats:italic><\/jats:styled-content> L. (mulberry) twig is known to have an inhibitory effect on pathogens in traditional Chinese medicine. In the present study, the dermophytic fungus, <jats:italic>Trichophyton rubrum<\/jats:italic>, was used to evaluate the inhibitory effect of total <jats:styled-content style=\"fixed-case\"><jats:italic>M. alba<\/jats:italic><\/jats:styled-content> twig extract and extracts obtained using solvents with different polarities by the method of 96\u2010well MTT colorimetry. The main active substance was isolated and identified by tracking its activity. In addition, the inhibitory effects of active extracts and a single active substance were investigated in combination with miconazole nitrate. Our data indicated that ethyl acetate extracts of mulberry twig (TEE) exhibited a desired inhibitory activity on <jats:styled-content style=\"fixed-case\"><jats:italic>T. rubrum<\/jats:italic><\/jats:styled-content> with the minimum inhibitory concentration (MIC) of 1.000\u00a0mg\/mL. With activity tracking, the main substance showing antimicrobial activity was oxyresveratrol (OXY), which was isolated from TEE. Its MIC for inhibiting the growth of <jats:styled-content style=\"fixed-case\"><jats:italic>T. rubrum<\/jats:italic><\/jats:styled-content> was 0.500\u00a0mg\/mL. The combined use of miconazole nitrate and OXY showed a synergistic inhibitory effect, as shown by a significant decrease in the MIC of both components. Based on the OXY content in TEE, the contribution rate of OXY to the inhibitory effect of TEE on <jats:styled-content style=\"fixed-case\"><jats:italic>T. rubrum<\/jats:italic><\/jats:styled-content> was 80.52%, so it was determined to be the main antimicrobial substance in <jats:styled-content style=\"fixed-case\"><jats:italic>M. alba<\/jats:italic><\/jats:styled-content> twig. Copyright \u00a9 2017 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/ptr.5926","type":"journal-article","created":{"date-parts":[[2017,10,11]],"date-time":"2017-10-11T06:46:47Z","timestamp":1507704407000},"page":"1842-1848","source":"Crossref","is-referenced-by-count":25,"title":["Oxyresveratrol, a Stilbene Compound from <scp><i>Morus alba<\/i><\/scp> L. Twig Extract Active Against <i>Trichophyton rubrum<\/i>"],"prefix":"10.1002","volume":"31","author":[{"given":"Hai\u2010Peng","family":"Lu","sequence":"first","affiliation":[{"name":"College of Biotechnology Southwest University  Chongqing 400715 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ya\u2010Nan","family":"Jia","sequence":"additional","affiliation":[{"name":"College of Biotechnology Southwest University  Chongqing 400715 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ya\u2010Lin","family":"Peng","sequence":"additional","affiliation":[{"name":"College of Biotechnology Southwest University  Chongqing 400715 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan","family":"Yu","sequence":"additional","affiliation":[{"name":"College of Biotechnology Southwest University  Chongqing 400715 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Si\u2010Long","family":"Sun","sequence":"additional","affiliation":[{"name":"College of Biotechnology Southwest University  Chongqing 400715 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Meng\u2010Ting","family":"Yue","sequence":"additional","affiliation":[{"name":"College of Biotechnology Southwest University  Chongqing 400715 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Min\u2010Hui","family":"Pan","sequence":"additional","affiliation":[{"name":"College of Biotechnology Southwest University  Chongqing 400715 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ling\u2010Shu","family":"Zeng","sequence":"additional","affiliation":[{"name":"Institute of Sericulture Science and Technology Research  Chongqing 400700 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5525-6298","authenticated-orcid":false,"given":"Li","family":"Xu","sequence":"additional","affiliation":[{"name":"College of Biotechnology Southwest University  Chongqing 400715 China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,10,11]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12906-015-0580-0"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/jsfa.7208"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02072-06"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fct.2010.11.045"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.2298\/JSC0303235D"},{"key":"e_1_2_7_7_1","first-page":"007","article-title":"Analysis on toxicity of 40% lesang (Chlorpyrifos) to Tetranychus cinnabarinus with SPSS","volume":"4","author":"Fuan TSW","year":"2005","journal-title":"Guangxi Sericulture"},{"key":"e_1_2_7_8_1","first-page":"245","article-title":"In vitro antifungal drug susceptibility of dermatophytes isolated from patients in Al\u2010Medina, Saudi Arabia","volume":"8","author":"Hanafy AM","year":"2012","journal-title":"Egypt J Exp Biol (Bot)"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.apsusc.2014.03.125"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00044-016-1762-0"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/myc.12317"},{"key":"e_1_2_7_12_1","first-page":"129","article-title":"Antioxidant compounds from twig of Morus alba","volume":"8","author":"Jin WY","year":"2002","journal-title":"Natural Product Sciences"},{"key":"e_1_2_7_13_1","unstructured":"JingL.2015.The antimicrobial activity of flourescent brightener combined with antifungal agents againstTrichophyton rubrum. 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