{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,28]],"date-time":"2026-03-28T12:19:04Z","timestamp":1774700344681,"version":"3.50.1"},"reference-count":34,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2017,10,18]],"date-time":"2017-10-18T00:00:00Z","timestamp":1508284800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Natural Science Foundation Research Project of Shaanxi Province","award":["2016JQ8005"],"award-info":[{"award-number":["2016JQ8005"]}]},{"name":"Special Fund for Basic Scientific Research of Central Colleges, Chang'an University","award":["310831161004"],"award-info":[{"award-number":["310831161004"]}]},{"name":"Special Fund for Basic Scientific Research of Central Colleges, Chang'an University","award":["310829161004"],"award-info":[{"award-number":["310829161004"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Phytotherapy Research"],"published-print":{"date-parts":[[2018,1]]},"abstract":"<jats:p>Radix Astragalus has been shown to exert beneficial effects regarding the prevention postmenopausal osteoporosis. However, its mechanism of action remains to be investigated. Calycosin, formononetin, and calycosin\u20107\u2010<jats:italic>O<\/jats:italic>\u2010\u03b2\u2010<jats:sc>d<\/jats:sc>\u2010glucoside are the main isoflavone constituents of Astragalus. In this study, the abilities of these 3 compounds to promote osteogenic function of osteoblasts were compared, and the structure\u2013activity relationships of these osteotrophic isoflavones were determined. Calycosin exhibited a greater effect than formononetin and calycosin\u20107\u2010<jats:italic>O<\/jats:italic>\u2010\u03b2\u2010<jats:sc>d<\/jats:sc>\u2010glucoside regarding improvements in osteogenic function of osteoblasts, as demonstrated by cell proliferation, alkaline phosphatase activity, collagen I and osteocalcin secretion, and the number and area of mineralized bone nodules. This suggests that calycosin may be better than formononetin and calycosin\u20107\u2010<jats:italic>O<\/jats:italic>\u2010\u03b2\u2010<jats:sc>d<\/jats:sc>\u2010glucoside at preserving bone mass. In addition, calycosin, formononetin, and calycosin\u20107\u2010<jats:italic>O<\/jats:italic>\u2010\u03b2\u2010<jats:sc>d<\/jats:sc>\u2010glucoside stimulate the expression of bone morphogenetic protein 2 and runt\u2010related transcription factor 2 proteins, which indicates that all 3 agents may promote the osteogenesis of osteoblasts via regulation of bone morphogenetic protein 2 expression. In conclusion, calycosin may be the best candidate, with higher osteogenic activity than formononetin and calycosin\u20107\u2010<jats:italic>O<\/jats:italic>\u2010\u03b2\u2010<jats:sc>d<\/jats:sc>\u2010glucoside. The higher osteogenic activity of calycosin could be attributable to the superiority of its chemical structure (a hydroxyl group at position C3 of Ring B and no glucosyl group).<\/jats:p>","DOI":"10.1002\/ptr.5955","type":"journal-article","created":{"date-parts":[[2017,10,18]],"date-time":"2017-10-18T07:32:50Z","timestamp":1508311970000},"page":"115-124","source":"Crossref","is-referenced-by-count":34,"title":["A comparative study on the effect of promoting the osteogenic function of osteoblasts using isoflavones from <scp>R<\/scp>adix <scp>A<\/scp>stragalus"],"prefix":"10.1002","volume":"32","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2990-3313","authenticated-orcid":false,"given":"Xianghe","family":"Kong","sequence":"first","affiliation":[{"name":"Chang'an University  Xi'an Shaanxi 710064 P. R. 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