{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T18:57:48Z","timestamp":1790017068954,"version":"4.0.1"},"reference-count":34,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2018,1,24]],"date-time":"2018-01-24T00:00:00Z","timestamp":1516752000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"National Science Center, Warsaw, Poland","award":["[project No: 2015\/17\/D\/NZ7\/00822]"],"award-info":[{"award-number":["[project No: 2015\/17\/D\/NZ7\/00822]"]}]},{"name":"Medical University of Lublin, Poland","award":["[project No: DS 440\/2014-2015"],"award-info":[{"award-number":["[project No: DS 440\/2014-2015"]}]},{"name":"Medical University of Lublin, Poland","award":["DS 440\/2016-2017]"],"award-info":[{"award-number":["DS 440\/2016-2017]"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Phytotherapy Research"],"published-print":{"date-parts":[[2018,5]]},"abstract":"<jats:p>Three curcuminoids: bisdemethoxycurcumin, demethoxycurcumin, and curcumin from turmeric were successfully separated by a high capacity solvent system composed of heptane: chloroform: methanol: water mixture (5: 6: 3: 2\u00a0<jats:italic>v<\/jats:italic>\/v\/<jats:italic>v<\/jats:italic>\/v) tailored for centrifugal partition chromatographs at<jats:italic>K<\/jats:italic>\u2010values of 0.504, 1.057, 1.644, respectively. These three ferulic acid derivatives obtained at a purity rate exceeding 95% were analysed by an HPLC\u2010MS spectrometer. Turmeric extract inhibited the proliferation\/viability of A549 human lung cancer, HT29 colon cancer, and T98G glioblastoma cell lines in (3\u2010[4,5\u2010dimethylthiazol\u20102\u2010yl]\u20102,5\u2010diphenyltetrazolium bromide) tetrazolium reduction assay (MTT). Single curcuminoids significantly decreased the viability\/proliferation of lung cancer cells in a dose\u2010dependent manner. However, total extract displayed the superior anticancer activity in the investigated cell lines. Crude extract in combination with cisplatin augmented the decrease in the viability of cancer cells compared with single compound treatment in A549 lung cancer cells. Total extract of<jats:styled-content style=\"fixed-case\"><jats:italic>Curcuma longa<\/jats:italic><\/jats:styled-content>could be regarded as being more effective against lung cancer cells in vitro than its separated compounds.<\/jats:p>","DOI":"10.1002\/ptr.6035","type":"journal-article","created":{"date-parts":[[2018,1,25]],"date-time":"2018-01-25T03:50:44Z","timestamp":1516852244000},"page":"933-942","source":"Crossref","is-referenced-by-count":53,"title":["Superior anticancer activity is demonstrated by total extract of<i>Curcuma longa<\/i>L. as opposed to individual curcuminoids separated by centrifugal partition chromatography"],"prefix":"10.1002","volume":"32","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7076-600X","authenticated-orcid":false,"given":"Wirginia","family":"Kukula\u2010Koch","sequence":"first","affiliation":[{"name":"Department of Pharmacognosy with Medicinal Plant Unit Medical University of Lublin 1 Chod\u017aki 20\u2010093 Lublin Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aneta","family":"Grabarska","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology Medical University of Lublin 1 Chod\u017aki 20\u2010093 Lublin Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jarogniew","family":"\u0141uszczki","sequence":"additional","affiliation":[{"name":"Department of Pathophysiology Medical University of Lublin 8 Jaczewskiego 20\u2010090 Lublin Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lidia","family":"Czernicka","sequence":"additional","affiliation":[{"name":"Department of Food and Nutrition Medical University of Lublin 4a Chod\u017aki. 20\u2010093 Lublin Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ewa","family":"Nowosadzka","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology Medical University of Lublin 1 Chod\u017aki 20\u2010093 Lublin Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ewelina","family":"Gumbarewicz","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology Medical University of Lublin 1 Chod\u017aki 20\u2010093 Lublin Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Agata","family":"Jarz\u0105b","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology Medical University of Lublin 1 Chod\u017aki 20\u2010093 Lublin Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gregoire","family":"Audo","sequence":"additional","affiliation":[{"name":"Armen Instrument Application Laboratory ZI Kermelin 56890 Saint Av\u00e9 France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shakti","family":"Upadhyay","sequence":"additional","affiliation":[{"name":"Immunocon Biotech Sp. z o. o. 39A Zana 20\u2010601 Lublin Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazimierz","family":"G\u0142owniak","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy with Medicinal Plant Unit Medical University of Lublin 1 Chod\u017aki 20\u2010093 Lublin Poland"},{"name":"Department of Cosmetology University of Information Technology and Management 2 Sucharskiego 35\u2010225 Rzesz\u00f3w Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrzej","family":"Stepulak","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology Medical University of Lublin 1 Chod\u017aki 20\u2010093 Lublin Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,1,24]]},"reference":[{"key":"e_1_2_8_2_1","first-page":"65","article-title":"Curcuma longa and curcumin","volume":"55","author":"Akram M.","year":"2010","journal-title":"Romanian Journal of Biochemistry\u2014Plant Biology"},{"key":"e_1_2_8_3_1","article-title":"Biological activities of curcuminoids, other biomolecules from turmeric and their derivatives\u2014A review","author":"Amalraja A.","year":"2016","journal-title":"African Journal of Traditional, Complementary, and Alternative Medicines"},{"issue":"6","key":"e_1_2_8_4_1","first-page":"1557","article-title":"Curcumin inhibits hypoxia\u2010induced angiogenesis via down\u2010regulation of HIF\u20101","volume":"15","author":"Bae M. 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