{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T05:22:32Z","timestamp":1789881752622,"version":"4.0.1"},"reference-count":34,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2018,11,12]],"date-time":"2018-11-12T00:00:00Z","timestamp":1541980800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"National Natural Scientific Fund","award":["81272987"],"award-info":[{"award-number":["81272987"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Phytotherapy Research"],"published-print":{"date-parts":[[2019,1]]},"abstract":"<jats:p>\n                    Puerarin is the major bioactive ingredient derived from the root of the\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Pueraria lobata<\/jats:italic>\n                    <\/jats:styled-content>\n                    (Willd.), and its antioxidative stress effects have been demonstrated in several previous studies. Moreover, Puerarin can upregulate melanin synthesis and microphthalmia\u2010associated transcription factor (MITF) transcription by increasing cAMP level of intracellular cyclic adenosine monophosphate. Vitiligo is an acquired cutaneous disorder of pigmentation, and the pathogenesis has remained elusive. Current treatment modalities are directed towards achieving repigmentation. In this study, we found that after treating with puerarin at various concentrations of 40\u00a0\u03bcmol\/L, the melanin content of human melanocytes increased significantly and the apparent level of protein and the RNA levels of MITF, tyrosinase (TYR), and tyrosinase\u2010related protein 1 (TRP\u20101) were also increased. Further, puerarin was shown to inhibit phosphorylation and activation of extracellular signal\u2010regulated kinase 1 and 2 (ERK1\/2) without significantly affecting p38 and c\u2010Jun N\u2010terminal kinase phosphorylation. These results demonstrated that puerarin stimulated melanogenesis in human melanocytes via inhibition of ERK1\/2 signaling pathways, which leads to upregulation of MITF and TYR as well as TRP\u20101 subsequently. Additionally, mice vitiligo models with puerarin treatment showed lighter pathological changes. Therefore, we suggested that puerarin might be a potential medicine for vitiligo.\n                  <\/jats:p>","DOI":"10.1002\/ptr.6218","type":"journal-article","created":{"date-parts":[[2018,11,13]],"date-time":"2018-11-13T01:25:51Z","timestamp":1542072351000},"page":"205-213","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Effect of puerarin on melanogenesis in human melanocytes and vitiligo mouse models and the underlying mechanism"],"prefix":"10.1002","volume":"33","author":[{"given":"Xiaoxia","family":"Ding","sequence":"first","affiliation":[{"name":"Department of Dermatology and Venereology The First Affiliated Hospital of Wenzhou Medical University Wenzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Enci","family":"Mei","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Venereology The First Affiliated Hospital of Wenzhou Medical University Wenzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Murong","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Venereology The First Affiliated Hospital of Wenzhou Medical University Wenzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chaofeng","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Urinary Surgery The First Affiliated Hospital of Wenzhou Medical University Wenzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaobin","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Orthopedics The Second Affiliated Hospital of Wenzhou Medical University Wenzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Cai","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Venereology The First Affiliated Hospital of Wenzhou Medical University Wenzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6063-6399","authenticated-orcid":false,"given":"Zhiming","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Venereology The First Affiliated Hospital of Wenzhou Medical University Wenzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,11,12]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M109.070573"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00383-013-3454-x"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1142\/S0192415X08005758"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1590\/abd1806-4841.20142717"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1177\/039463201402700403"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.jidsp.5640176"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jdermsci.2004.08.014"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1734-1140(11)70590-4"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biochi.2012.01.018"},{"issue":"16","key":"e_1_2_7_11_1","doi-asserted-by":"crossref","first-page":"2966","DOI":"10.3760\/cma.j.issn.0366-6999.20140314","article-title":"Effect and mechanism of tacrolimus on melanogenesis on A375 human melanoma cells","volume":"127","author":"Huang H.","year":"2014","journal-title":"Chinese Medical Journal"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.3109\/13880209.2013.771376"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.taap.2008.09.006"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0749.2003.00072.x"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1248\/bpb.30.180"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/pcmr.12298"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/exd.12103"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molmed.2006.07.008"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-1097.2012.01138.x"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.2165\/00044011-200626050-00001"},{"key":"e_1_2_7_21_1","first-page":"919","article-title":"Signaling pathways mediating melanogenesis","volume":"45","author":"Park H. 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