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(Malvaceae) is a medicinal plant widely found in Southern Brazil, and popularly used for inflammatory disorders and to pain relief. A phytochemical analysis followed by an investigation about antinociceptive potential and mechanism of action were performed with leaves and roots extracts. Methanolic extracts, designated as\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>S.\u00a0tuberculata<\/jats:italic>\n                    <\/jats:styled-content>\n                    leaves extract (STLE) and\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>S.\u00a0tuberculata<\/jats:italic>\n                    <\/jats:styled-content>\n                    roots extract, were analyzed both by UHPLC\u2013MS. The in vivo antinociceptive potential of STLE (10\u2013300\u00a0mg\u00a0kg\n                    <jats:sup>\u22121<\/jats:sup>\n                    ) was assessed in mice subjected to the acetic acid\u2010induced abdominal writhes and formalin model. Agonist\/antagonist tests and computational docking suggest the involvement of opioid and adenosinergic systems. The main chemical class detected on extracts was the ecdysteroids, and 20\u2010hydoxyecdysone (20HE) was confirmed as the major phytoconstituent. The pretreatment with STLE (100\u00a0mg\u00a0kg\n                    <jats:sup>\u22121<\/jats:sup>\n                    ) reduced more than 70% abdominal contortions induced by acetic acid model and produced significant inhibition on formalin\u2010induced licking response. The mechanism of action study revealed STLE might act through opioid and adenosine systems. Molecular docking suggested kaempferol derivative and 20HE might interacting with \u03bc\u2010opioid receptor. Thus, the results suggest the existence of antinociceptive potential from\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>S.\u00a0tuberculata<\/jats:italic>\n                    <\/jats:styled-content>\n                    extracts being in accordance to the traditional use.\n                  <\/jats:p>","DOI":"10.1002\/ptr.6220","type":"journal-article","created":{"date-parts":[[2018,10,30]],"date-time":"2018-10-30T06:16:16Z","timestamp":1540880176000},"page":"224-233","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["<i>Sida tuberculata<\/i>\n                    extract reduces the nociceptive response by chemical noxious stimuli in mice: Implications for mechanism of action, relation to chemical composition and molecular docking"],"prefix":"10.1002","volume":"33","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8474-5100","authenticated-orcid":false,"given":"Hemerson S.","family":"da Rosa","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Bioqu\u00edmica e Toxicologia de Produtos Naturais e Sint\u00e9ticos Universidade Federal do Pampa  Uruguaiana Brazil"},{"name":"Laborat\u00f3rio de Desenvolvimento e Controle de Qualidade em Medicamentos Universidade Federal do Pampa  Uruguaiana Brazil"},{"name":"Programa de P\u00f3s\u2010gradua\u00e7\u00e3o em Bioqu\u00edmica Universidade Federal do Pampa  Uruguaiana Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Igor S.","family":"Coelho","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s\u2010Gradua\u00e7\u00e3o em Neuroci\u00eancias, Centro de Ci\u00eancias Biol\u00f3gicas Universidade Federal de Santa Catarina  Florian\u00f3polis Brazil"},{"name":"Laborat\u00f3rio de Neurobiologia da Dor e Inflama\u00e7\u00e3o, Departamento de Ci\u00eancias Fisiol\u00f3gicas, Centro de Ci\u00eancias Biol\u00f3gicas Universidade Federal de Santa Catarina  Florian\u00f3polis Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Morgana Duarte","family":"da Silva","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s\u2010gradua\u00e7\u00e3o em Bioqu\u00edmica Universidade Federal do Pampa  Uruguaiana Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariana S.","family":"Fernandes","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Desenvolvimento e Controle de Qualidade em Medicamentos Universidade Federal do Pampa  Uruguaiana Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pablo Ricardo","family":"Bertelli","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biotecnologia de Produtos Naturais e Sint\u00e9ticos, Instituto de Biotecnologia Universidade de Caxias do Sul (UCS)  Caxias do Sul Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luciane","family":"Minetto","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biotecnologia de Produtos Naturais e Sint\u00e9ticos, Instituto de Biotecnologia Universidade de Caxias do Sul (UCS)  Caxias do Sul Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sidnei","family":"Moura","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biotecnologia de Produtos Naturais e Sint\u00e9ticos, Instituto de Biotecnologia Universidade de Caxias do Sul (UCS)  Caxias do Sul Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F\u00e1vero","family":"de Paula","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Desenvolvimento e Controle de Qualidade em Medicamentos Universidade Federal do Pampa  Uruguaiana Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6435-4698","authenticated-orcid":false,"given":"Adair R.","family":"Santos","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s\u2010Gradua\u00e7\u00e3o em Neuroci\u00eancias, Centro de Ci\u00eancias Biol\u00f3gicas Universidade Federal de Santa Catarina  Florian\u00f3polis Brazil"},{"name":"Laborat\u00f3rio de Neurobiologia da Dor e Inflama\u00e7\u00e3o, Departamento de Ci\u00eancias Fisiol\u00f3gicas, Centro de Ci\u00eancias Biol\u00f3gicas Universidade Federal de Santa Catarina  Florian\u00f3polis Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreas S. 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