{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T22:38:59Z","timestamp":1783636739569,"version":"3.55.0"},"reference-count":30,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2019,1,10]],"date-time":"2019-01-10T00:00:00Z","timestamp":1547078400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100005401","name":"Ministero delle Politiche Agricole Alimentari e Forestali","doi-asserted-by":"publisher","award":["Part of the work (isolation of natural erucin) has"],"award-info":[{"award-number":["Part of the work (isolation of natural erucin) has"]}],"id":[{"id":"10.13039\/501100005401","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Phytotherapy Research"],"published-print":{"date-parts":[[2019,3]]},"abstract":"<jats:p>Plants of the <jats:italic>Brassicaceae<\/jats:italic> family are well\u2010known for containing the glucosinolate myrosinase system, which is able to release isothiocyanates after plant biotic and abiotic lesions. Erucin (ERU; 1\u2010isothiocyanato\u20104\u2010(methylthio)\u2010butane), an isothiocyanate particularly abundant in arugula (<jats:styled-content style=\"fixed-case\"><jats:italic>Eruca sativa<\/jats:italic><\/jats:styled-content> Mill., <jats:styled-content style=\"fixed-case\"><jats:italic>Eruca vesicaria<\/jats:italic><\/jats:styled-content> L., etc.), derives from the hydrolysis of the glucosinolate glucoerucin by the enzyme myrosinase. Many other natural isothiocyanates influence cancer cells and, in particular, induce antiproliferative effects at relatively high concentrations. Similar antiproliferative effects have also been shown by the newly emerging gasotransmitter hydrogen sulfide (H<jats:sub>2<\/jats:sub>S) and by H<jats:sub>2<\/jats:sub>S\u2010releasing compounds. In a previous study, our group demonstrated that isothiocyanates release H<jats:sub>2<\/jats:sub>S in biological environments. In this work, we demonstrated the H<jats:sub>2<\/jats:sub>S\u2010donor properties of ERU in pancreatic adenocarcinoma cells (AsPC\u20101) and delineated its profile as a chemopreventive or anticancer agent. Indeed, ERU showed significant antiproliferative effects: ERU inhibited AsPC\u20101 cell viability at relatively high concentrations (30\u2013100\u00a0\u03bcM). Moreover, ERU inhibited cell migration, altered the AsPC\u20101 cell cycle, and exhibited proapoptotic effects. Finally, ERU inhibited ERK1\/2 phosphorylation. This mechanism is particularly important in AsPC\u20101 cells because they are characterized by a mutation in KRAS that determines KRAS hyperactivation followed by MAP\u2010kinase hyperphosphorylation, which plays a pivotal role in pancreatic cancer proliferation, growth, and survival.<\/jats:p>","DOI":"10.1002\/ptr.6278","type":"journal-article","created":{"date-parts":[[2019,1,11]],"date-time":"2019-01-11T10:06:02Z","timestamp":1547201162000},"page":"845-855","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":77,"title":["Anticancer properties of erucin, an H<sub>2<\/sub>S\u2010releasing isothiocyanate, on human pancreatic adenocarcinoma cells (AsPC\u20101)"],"prefix":"10.1002","volume":"33","author":[{"given":"Valentina","family":"Citi","sequence":"first","affiliation":[{"name":"Department of Pharmacy University of Pisa  Pisa 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Child Health \u2013 NEUROFARBA \u2013 Section of Pharmacology and Toxicology  Florence Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lara","family":"Testai","sequence":"additional","affiliation":[{"name":"Department of Pharmacy University of Pisa  Pisa Italy"},{"name":"Interdepartmental Research Centre \u201cNutraceuticals and Food for Health (NUTRAFOOD)\u201d University of Pisa  Pisa Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carla","family":"Ghelardini","sequence":"additional","affiliation":[{"name":"Department of Neuroscience, Psychology Drug Research and Child Health \u2013 NEUROFARBA \u2013 Section of Pharmacology and Toxicology  Florence Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luca","family":"Lazzeri","sequence":"additional","affiliation":[{"name":"Council for Agricultural Research and Economics, Research Centre for Cereal and Industrial Crops  Bologna 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Malaguti L. Cinti S. Ugolini L. De Nicola G. R. Bagatta M. \u2026D'Avino L.(2013).The Brassicaceae biofumigation system for plant cultivation and defence. An Italian twenty\u2010year experience of study and application.","DOI":"10.17660\/ActaHortic.2013.1005.44"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.orglett.5b01962"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201104305"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.3892\/ijo.2014.2305"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/ptr.6159"},{"issue":"6","key":"e_1_2_9_24_1","first-page":"2969","article-title":"Anticancer effect of exogenous hydrogen sulfide in cisplatinresistant A549\/DDP cells","volume":"39","author":"Ma Y.","year":"2018","journal-title":"Oncology Reports"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vph.2013.11.003"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.3390\/toxins2040593"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf000191p"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/pcmr.12312"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/chem.201303757"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9861(90)90346-Z"},{"issue":"4","key":"e_1_2_9_31_1","first-page":"4269","article-title":"In vitro studies of phenethyl isothiocyanate against the growth of LN229 human glioma cells","volume":"8","author":"Su J. 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