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The aim of this study was to investigate the antimicrobial and resistance modifying activity of <jats:styled-content style=\"fixed-case\"><jats:italic>N.\u00a0sativa<\/jats:italic><\/jats:styled-content> EO, thymoquinone, carvacrol, and p\u2010cymene against one methicillin susceptible and one methicillin resistant <jats:italic>Staphylococcus aureus<\/jats:italic> strain. <jats:styled-content style=\"fixed-case\"><jats:italic>N.\u00a0sativa<\/jats:italic><\/jats:styled-content> EO, thymoquinone, carvacrol, and p\u2010cymene were assessed for antimicrobial activity and modulation of antimicrobial resistance (by broth microdilution), inhibition of antimicrobial efflux (by ethidium bromide [EtBr] accumulation assay), relative expression of <jats:italic>mepA<\/jats:italic> gene (by real\u2010time reverse transcriptase quantitative polymerase chain reaction), membrane disrupting effect (by LIVE\/DEAD BacLight\u2122 Kit), and finally antibiofilm activity (by the crystal violet assay). Both strains of <jats:italic>S.\u00a0aureus<\/jats:italic> were susceptible to <jats:styled-content style=\"fixed-case\"><jats:italic>N.\u00a0sativa<\/jats:italic><\/jats:styled-content> EO, thymoquinone, and carvacrol. <jats:styled-content style=\"fixed-case\"><jats:italic>N.\u00a0sativa<\/jats:italic><\/jats:styled-content> EO and carvacrol induced the increase of EtBr accumulated by both <jats:italic>S.\u00a0aureus<\/jats:italic> strains. Membrane integrity of ATCC strain was disrupted by carvacrol and p\u2010cymene, whereas for the methicillin resistant <jats:italic>S.\u00a0aureus<\/jats:italic> (MRSA) strain the membrane integrity was disrupted by each compound. <jats:styled-content style=\"fixed-case\"><jats:italic>N.\u00a0sativa<\/jats:italic><\/jats:styled-content> EO and its bioactive compounds such as carvacrol and p\u2010cymene could be applied as resistance modifiers in MRSA strains.<\/jats:p>","DOI":"10.1002\/ptr.6294","type":"journal-article","created":{"date-parts":[[2019,1,24]],"date-time":"2019-01-24T12:02:39Z","timestamp":1548331359000},"page":"1010-1018","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":78,"title":["<i>Nigella sativa<\/i> essential oil and its bioactive compounds as resistance modifiers against <i>Staphylococcus aureus<\/i>"],"prefix":"10.1002","volume":"33","author":[{"given":"Ahmad","family":"Mouwakeh","sequence":"first","affiliation":[{"name":"Department of Microbiology and Biotechnology Szent Istv\u00e1n University  Budapest Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annam\u00e1ria","family":"Kincses","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Immunobiology University of Szeged  Szeged Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M\u00e1rta","family":"Nov\u00e9","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Immunobiology University of Szeged  Szeged Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T\u00edmea","family":"Mosolyg\u00f3","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Immunobiology University of Szeged  Szeged Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Csilla","family":"Moh\u00e1csi\u2010Farkas","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Biotechnology Szent Istv\u00e1n University  Budapest Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3344-5308","authenticated-orcid":false,"given":"Gabriella","family":"Kisk\u00f3","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Biotechnology Szent Istv\u00e1n University  Budapest Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8085-0950","authenticated-orcid":false,"given":"Gabriella","family":"Spengler","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Immunobiology University of Szeged  Szeged Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,1,23]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.etap.2017.12.017"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1556\/1886.2017.00001"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj20020957"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.2010.04740.x"},{"issue":"29","key":"e_1_2_7_6_1","first-page":"1","article-title":"Antibacterial activity of Thymoquinone, an active principle of Nigella sativa and its potency to prevent bacterial biofilm formation","volume":"11","author":"Chaieb K.","year":"2011","journal-title":"Complementary & Alternative Medicine"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkn217"},{"issue":"6","key":"e_1_2_7_8_1","first-page":"1985","article-title":"Antimicrobial activity of Nigella sativa L. seed oil against multi\u2010drug resistant Staphylococcus aureus isolated from diabetic wounds","volume":"28","author":"Emeka L. 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