{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,22]],"date-time":"2026-06-22T23:03:42Z","timestamp":1782169422732,"version":"3.54.5"},"reference-count":36,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2019,2,15]],"date-time":"2019-02-15T00:00:00Z","timestamp":1550188800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81560213"],"award-info":[{"award-number":["81560213"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Phytotherapy Research"],"published-print":{"date-parts":[[2019,4]]},"abstract":"<jats:p>Osteoporosis is characterized by low bone mineral density and microarchitectural deterioration of bone tissue. N\u2010(3\u2010methoxybenzyl)\u2010(9Z,12Z,15Z)\u2010octadecatrienamide (MBOC) is one of the macamides isolated from Maca (<jats:styled-content style=\"fixed-case\"><jats:italic>Lepidium meyenii<\/jats:italic><\/jats:styled-content> Walp.), a cruciferous plant from the Andes of Peru. In this study, C3H\/10T1\/2 mesenchymal stem cells were treated with MBOC in osteogenic induction medium. An ovariectomized (OVX) mouse model was used to investigate the effect of 1\u2010month MBOC treatment on the prevention of postmenopausal osteoporosis. Remarkably, trabecular thickness, trabecular number, and bone volume\/tissue volume of the distal femoral metaphysis were significantly increased in OVX\u00a0+\u00a0MBOC mice compared with OVX mice, as revealed by microcomputed tomography analysis. Trabecular separation was decreased in OVX\u00a0+\u00a0MBOC mice compared with OVX mice. Consistently, MBOC increased the levels of osteocalcin and runt\u2010related transcription factor 2 in OVX mice, as well as the expression of runt\u2010related transcription factor 2, osterix, and alkaline phosphatase in C3H\/10T1\/2 cells. Mechanistically, MBOC activates the canonical Wnt\/\u03b2\u2010catenin signaling pathway via inhibiting phosphorylation of GSK\u20103\u03b2 at Tyr216 and maintaining \u03b2\u2010catenin expression. Collectively, the current study demonstrates the robustness of MBOC in the induction of mesenchymal stem cells osteogenic differentiation and consequent bone formation, suggesting that MBOC may be a potentially effective drug to treat postmenopausal osteoporosis.<\/jats:p>","DOI":"10.1002\/ptr.6301","type":"journal-article","created":{"date-parts":[[2019,2,15]],"date-time":"2019-02-15T10:47:00Z","timestamp":1550227620000},"page":"1074-1083","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["N\u2010(3\u2010methoxybenzyl)\u2010(9Z,12Z,15Z)\u2010octadecatrienamide promotes bone formation via the canonical Wnt\/\u03b2\u2010catenin signaling pathway"],"prefix":"10.1002","volume":"33","author":[{"given":"Ting","family":"Wang","sequence":"first","affiliation":[{"name":"Key Laboratory of Mental Health of the Ministry of Education, Department of Cell Biology School of Basic Medical Sciences, Southern Medical University  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