{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T01:24:45Z","timestamp":1789521885108,"version":"build-2803163510"},"reference-count":36,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2019,6,17]],"date-time":"2019-06-17T00:00:00Z","timestamp":1560729600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Basic Research Program of China","doi-asserted-by":"publisher","award":["2016YFD0501309"],"award-info":[{"award-number":["2016YFD0501309"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Phytotherapy Research"],"published-print":{"date-parts":[[2019,8]]},"abstract":"<jats:p>Vancomycin is a glycopeptide antibiotic widely used to treat infections caused by methicillin\u2010resistant <jats:styled-content style=\"fixed-case\"><jats:italic>Staphylococcus aureus<\/jats:italic><\/jats:styled-content>. However, nephrotoxicity is a major adverse side effect, and the development of effective nephroprotective agents remains a priority in antimicrobial chemotherapy. In this study, we investigated the cell protective effects of the flavonol glycoside rutin against vancomycin\u2010induced toxicity. Vancomycin added to porcine renal tubular LLC\u2010PK1 cells caused an increase of production of intracellular reactive oxygen species and subsequent apoptotic cell death. Pretreatment of LLC\u2010PK1 cells with rutin at 5, 10, and 20\u00a0\u03bcM for 2\u00a0hr prior to 2\u2010mM vancomycin exposure for 24\u00a0hr significantly decreased intracellular reactive oxygen species and increased superoxide dismutase and catalase activities. Rutin pretreatment also protected cells from vancomycin\u2010induced caspase activation, mitochondrial membrane depolarization, and subsequent apoptosis. This study demonstrates a protective effect of rutin and suggests that rutin coadministration is an alternative therapy for treatment of vancomycin\u2010induced nephrotoxicity.<\/jats:p>","DOI":"10.1002\/ptr.6391","type":"journal-article","created":{"date-parts":[[2019,6,18]],"date-time":"2019-06-18T05:09:04Z","timestamp":1560834544000},"page":"2056-2063","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":40,"title":["Rutin attenuates vancomycin\u2010induced renal tubular cell apoptosis via suppression of apoptosis, mitochondrial dysfunction, and oxidative stress"],"prefix":"10.1002","volume":"33","author":[{"given":"Shaoqi","family":"Qu","sequence":"first","affiliation":[{"name":"Agricultural Bio\u2010pharmaceutical Laboratory Qingdao Agricultural University  Qingdao 266109 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cunchun","family":"Dai","sequence":"additional","affiliation":[{"name":"Agricultural Bio\u2010pharmaceutical Laboratory Qingdao Agricultural University  Qingdao 266109 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Guo","sequence":"additional","affiliation":[{"name":"Agricultural Bio\u2010pharmaceutical Laboratory Qingdao Agricultural University  Qingdao 266109 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cuncai","family":"Wang","sequence":"additional","affiliation":[{"name":"Agricultural Bio\u2010pharmaceutical Laboratory Qingdao Agricultural University  Qingdao 266109 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7282-2340","authenticated-orcid":false,"given":"Zhihui","family":"Hao","sequence":"additional","affiliation":[{"name":"Agricultural Bio\u2010pharmaceutical Laboratory Qingdao Agricultural University  Qingdao 266109 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qihe","family":"Tang","sequence":"additional","affiliation":[{"name":"College of Chemistry and Pharmaceutical Sciences Qingdao Agricultural University  Qingdao China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haixia","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Chemistry and Pharmaceutical Sciences Qingdao Agricultural University  Qingdao China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanping","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Chemistry and Pharmaceutical Sciences Qingdao Agricultural University  Qingdao China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,6,17]]},"reference":[{"issue":"2","key":"e_1_2_7_2_1","first-page":"80","article-title":"Citrus, rutin and on their vein permeability effects","volume":"3","author":"Altinterim B.","year":"2014","journal-title":"RJAEM."},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.2174\/1381612823666170329142936"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2012.02.038"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.02432-16"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.34.1.139"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00168-11"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tox.2017.04.015"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1021\/cb4007024"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12035-016-0276-6"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxlet.2009.10.002"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/toxsci\/kfn203"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2016.04.003"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkx153"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00228-012-1259-9"},{"key":"e_1_2_7_16_1","doi-asserted-by":"crossref","first-page":"B47","DOI":"10.1053\/j.ajkd.2008.02.085","article-title":"79: Acute interstitial nephritis (AIN) secondary to vancomycin. 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