{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T22:31:26Z","timestamp":1791844286328,"version":"4.3.4"},"reference-count":27,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2019,7,18]],"date-time":"2019-07-18T00:00:00Z","timestamp":1563408000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Phytotherapy Research"],"published-print":{"date-parts":[[2019,9]]},"abstract":"<jats:p>Lupus nephritis (LN) is an autoimmune disease caused by systemic lupus erythematosus. Excessive proliferation of mesangial cells is one of the most serious pathological manifestations of LN. In addition, the expression of PTX3 is elevated in the serum of patients with LN. Quercetin has good anti\u2010inflammatory effects and immunomodulatory activities. In this study, the result of MTT indicated that quercetin treatment alleviated the excessive proliferation of mesangial cells. ELISA and immunofluorescence experiments showed that quercetin treatment inhibited the expression of PTX3. Three doses of quercetin (20, 40, and 80\u00a0\u03bcM) were selected for the experiment. It is noteworthy that the efficacy of quercetin at 80\u00a0\u03bcM was significantly better than that of other dose groups. And the effect in inhibiting PTX3 expression was comparable with that of the PDTC (80\u00a0\u03bcM) positive control. Western blot and qRT\u2010PCR analysis revealed that quercetin treatment reduced the expression of nuclear factor\u2010\u03baB p65 and IKK\u03b2, increased the expression of I\u03baB\u03b1, and inhibited the expression of PTX3. In conclusion, through inhibiting the activation of nuclear factor\u2010\u03baB signaling pathway, quercetin treatment could reduce the expression of PTX3 and inhibit the excessive proliferation of mesangial cells, suggesting that quercetin is a potential therapeutic drug for LN.<\/jats:p>","DOI":"10.1002\/ptr.6430","type":"journal-article","created":{"date-parts":[[2019,7,18]],"date-time":"2019-07-18T08:53:00Z","timestamp":1563439980000},"page":"2401-2408","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["Quercetin reduces TNF\u2010\u03b1\u2010induced mesangial cell proliferation and inhibits PTX3 production: Involvement of NF\u2010\u03baB signaling pathway"],"prefix":"10.1002","volume":"33","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3378-9071","authenticated-orcid":false,"given":"Yue","family":"Liu","sequence":"first","affiliation":[{"name":"School of Pharmacy, Key Laboratory of Molecular Pharmacology and 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