{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T07:00:50Z","timestamp":1787900450505,"version":"build-2784847793"},"reference-count":24,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2019,7,25]],"date-time":"2019-07-25T00:00:00Z","timestamp":1564012800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Phytotherapy Research"],"published-print":{"date-parts":[[2019,9]]},"abstract":"<jats:p>The pollen extract Cernitin\u00ae is widely used for treatment of benign prostatic hyperplasia (BPH) and non\u2010bacterial chronin prostatitis. However, little is known about the underlying molecular mechanisms to explain the clinical effects of Cernitin\u00ae. In this study, we sought to investigate the cellular mechanisms by which Cernitin\u00ae induces its effects on human prostatic cell lines BPH\u20101 and WPMY\u20101 and primary human peripheral blood mononuclear cells (hPBMCs) in vitro. We examined the effects of Cernitin\u00ae formulas T60 and GBX on the protein expression, proliferation, and cytokines production. Results revealed that Cernitin\u00ae upregulated antiinflammatory cytokine interleukin (IL)\u201010 and its receptors IL\u201010RA and IL\u201010B in addition to the upregulation of tumour necrosis factor\u2010related apoptosis\u2010inducing ligand in hPBMC. Interestingly, the levels of proinflammatory cytokines IL\u20106 and IL\u20108 were also increased. Furthermore, Cernitin\u00ae had significantly increased the level of IL\u201010 in BPH\u20101 and WPMY\u20101 cells. The level of IL\u20106 was also significantly increased in these cells although both T60 and GBX inhibited STAT\u20103 phosphorylation. Moreover, Cernitin\u00ae formulas had significantly reduced androgen receptor and prostate\u2010specific antigen protein expression in stromal cells (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05). Treatment with GBX and T60 had significantly inhibited proliferation of BPH (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.001) and stromal cells (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05), in a dose\u2010dependent manner. Taken together, treatment with Cernitin\u00ae showed to regulate cytokines level in both prostatic cell lines and hPBMCs and it was associated with decreased androgen receptor and prostate\u2010specific antigen levels WPMY\u20101 cells.<\/jats:p>","DOI":"10.1002\/ptr.6438","type":"journal-article","created":{"date-parts":[[2019,7,25]],"date-time":"2019-07-25T07:29:43Z","timestamp":1564039783000},"page":"2457-2464","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["The effects of Cernitin\u00ae on inflammatory parameters and benign prostatic hyperplasia: An in vitro study"],"prefix":"10.1002","volume":"33","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5738-4688","authenticated-orcid":false,"given":"Nishtman","family":"Dizeyi","sequence":"first","affiliation":[{"name":"Department of Translational Medicine Lund University  Malm\u00f6 Sweden"},{"name":"Preclinical Research AB Cernelle  \u00c4ngelholm 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