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The anxiolytic activity at 100, 200, and 400\u2009mg\/Kg doses were assessed in mice using elevated plus maze (EPM) and light\/dark transition (LDT) test models on days 1 and 16. Neurotransmitters such as monoamines (serotonin, norepinephrine, and dopamine), \u03b3\u2010aminobutyric acid (GABA), and glutamate were estimated in different regions of the brain (cortex, hippocampus, and cerebellum and brain stem). Phytoconstituents identified using gas chromatography\u2013mass spectrometry and liquid chromatography\u2013mass spectrometry were analyzed in silico to evaluate their potential binding mode(s) to GABA<jats:sub>A<\/jats:sub> and 5\u2010HT<jats:sub>1A<\/jats:sub> receptors. Phytochemical studies showed the presence of alkaloids, tannins, flavonoids, saponins, anthraquinone glycosides, carbohydrates, and proteins, whereas total flavonoid and phenol contents were estimated to be 64.96\u2009\u00b1\u20090.95 and 206.58\u2009\u00b1\u20091.60\u2009mg\/g of dried extract, respectively. PLE treatment significantly enhanced exploratory activity of mice in EPM and LDT models with significant effects on monoamines, GABA and glutamate levels in the brain. The in silico studies suggested the interaction(s) of PLE component(s) with GABA<jats:sub>A<\/jats:sub>\/5\u2010HT<jats:sub>1A<\/jats:sub> receptors as a potential mechanism of its anxiolytic activity.<\/jats:p>","DOI":"10.1002\/ptr.6900","type":"journal-article","created":{"date-parts":[[2020,10,9]],"date-time":"2020-10-09T09:51:13Z","timestamp":1602237073000},"page":"1399-1415","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Anxiolytic activity of <i>Psidium guajava<\/i> in mice subjected to chronic restraint stress and effect on neurotransmitters in brain"],"prefix":"10.1002","volume":"35","author":[{"given":"Swati","family":"Sahoo","sequence":"first","affiliation":[{"name":"Sunandan Divatia School of Science NMIMS (Deemed\u2010to\u2010be) University  Mumbai India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Prashant S.","family":"Kharkar","sequence":"additional","affiliation":[{"name":"Shobhaben Pratapbhai Patel School of Pharmacy and Technology Management SVKM's NMIMS  Mumbai India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Niteshkumar U.","family":"Sahu","sequence":"additional","affiliation":[{"name":"Shobhaben Pratapbhai Patel School of Pharmacy and Technology Management SVKM's NMIMS  Mumbai India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5948-7082","authenticated-orcid":false,"given":"Brijesh","family":"S.","sequence":"additional","affiliation":[{"name":"Sunandan Divatia School of Science NMIMS (Deemed\u2010to\u2010be) University  Mumbai India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,10,9]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3940(93)90200-5"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.3109\/10253891003667862"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.2174\/18715273113129990102"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrn4028"},{"key":"e_1_2_7_6_1","first-page":"94","article-title":"St. John's wort modulates brain regional serotonin metabolism in swim stressed rats","volume":"22","author":"Ara I.","year":"2009","journal-title":"Pakistan Journal of Pharmaceutical Sciences"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.physbeh.2014.06.003"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1636-07.2007"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/28.1.235"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pnpbp.2010.04.025"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1186\/1472-6882-10-33"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.2042-7158.1990.tb07059.x"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpsyg.2015.01520"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1472-8206.2007.00526.x"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.13-03-00914.1993"},{"issue":"3","key":"e_1_2_7_16_1","first-page":"157","article-title":"Effects of ethanolic leaf extract of Psidium guajava Linn. on l\u2010arginine induced obsessive compulsive disorder in mice","volume":"4","author":"Chinnala K. 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