{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T15:23:14Z","timestamp":1781796194382,"version":"3.54.5"},"reference-count":39,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2025,7,27]],"date-time":"2025-07-27T00:00:00Z","timestamp":1753574400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100017700","name":"Henan Provincial Science and Technology Research Project","doi-asserted-by":"publisher","award":["242102310536"],"award-info":[{"award-number":["242102310536"]}],"id":[{"id":"10.13039\/501100017700","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100017634","name":"Henan University of Chinese Medicine","doi-asserted-by":"publisher","award":["MP2024\u201006"],"award-info":[{"award-number":["MP2024\u201006"]}],"id":[{"id":"10.13039\/100017634","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Phytotherapy Research"],"published-print":{"date-parts":[[2025,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Reniformin A (RA) is a natural compound extracted from the medicinal herb <jats:italic>Isodon excisoides<\/jats:italic>, known for its tumor\u2010suppressive properties in lung cancer. Yet, its effects and mechanisms of action in other cancers, such as triple\u2010negative breast cancer (TNBC), remain unclear. This study aims to investigate the potential effects and underlying molecular mechanisms of RA in TNBC. Here, we demonstrate the significant anti\u2010cancer activity of RA against TNBC, primarily through the induction of mitochondrial dysfunction and intrinsic apoptosis. Molecular docking and in\u00a0vitro validation revealed that RA interacts directly with DRP1 at two primary binding sites. This interaction promotes the association of DRP1 with BAX, facilitating their translocation to mitochondria, where they trigger mitochondrial permeabilization, leading to the release of cytochrome c and subsequent apoptosis. Additionally, DRP1 is essential for RA\u2010induced apoptosis; disruption of the RA\u2010DRP1 interaction not only impeded the mitochondrial translocation of DRP1 and BAX but also significantly reduced RA's impact on mitochondrial function, apoptosis, and TNBC progression. The inhibition of the RA\u2010DRP1 interaction also compromised the activation of apoptosis and diminished the effectiveness of RA as a chemotherapeutic agent in\u00a0vivo. Collectively, these findings suggest that Reniformin A significantly inhibits TNBC by inducing DRP1\/BAX\u2010mediated apoptosis, offering a promising therapeutic strategy for TNBC treatment.<\/jats:p>","DOI":"10.1002\/ptr.70034","type":"journal-article","created":{"date-parts":[[2025,7,28]],"date-time":"2025-07-28T03:03:21Z","timestamp":1753671801000},"page":"4011-4027","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Reniformin A Suppresses Triple\u2010Negative Breast Cancer Progression by Inducing <scp>DRP1<\/scp>\u2010Mediated Mitochondrial Dysfunction and Apoptosis"],"prefix":"10.1002","volume":"39","author":[{"given":"Yifei","family":"Guan","sequence":"first","affiliation":[{"name":"Beijing Key Laboratory of Environmental and Viral Oncology, Beijing International Science and Technology Cooperation Base for Antiviral Drugs, College of Chemistry and Life Science Beijing University of Technology  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Comprehensive Treatment 2nd Medical Center of Chinese PLA General Hospital  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Radiology Seventh Medical Center of Chinese PLA General Hospital  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ran","family":"Zheng","sequence":"additional","affiliation":[{"name":"Beijing Key Laboratory of Environmental and Viral Oncology, Beijing International Science and Technology Cooperation Base for Antiviral Drugs, College of Chemistry and Life Science Beijing University of Technology  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pinghao","family":"Lin","sequence":"additional","affiliation":[{"name":"Beijing Key Laboratory of Environmental and Viral Oncology, Beijing International Science and Technology Cooperation Base for Antiviral Drugs, College of Chemistry and Life Science Beijing University of Technology  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yalin","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Pharmacy Henan University of Chinese Medicine  Zhengzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siyu","family":"Chen","sequence":"additional","affiliation":[{"name":"School of Pharmacy Henan University of Chinese Medicine  Zhengzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huiyu","family":"Zhu","sequence":"additional","affiliation":[{"name":"School of Pharmacy Henan University of Chinese Medicine  Zhengzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinhui","family":"Liu","sequence":"additional","affiliation":[{"name":"Beijing Key Laboratory of Environmental and Viral Oncology, Beijing International Science and Technology Cooperation Base for Antiviral Drugs, College of Chemistry and Life Science Beijing University of Technology  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9881-4197","authenticated-orcid":false,"given":"Hua","family":"Huang","sequence":"additional","affiliation":[{"name":"Beijing Key Laboratory of Environmental and Viral Oncology, Beijing International Science and Technology Cooperation Base for Antiviral Drugs, College of Chemistry and Life Science Beijing University of Technology  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,7,27]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41580-019-0173-8"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neures.2011.02.005"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41571-020-0341-y"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chemphyslip.2014.04.001"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e16-05-0286"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules200917544"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1080\/14786419.2017.1419225"},{"key":"e_1_2_9_9_1","first-page":"3361","article-title":"Studies on Chemical Constituents of Isodon Excisoides","volume":"41","author":"Dai L. 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