{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,15]],"date-time":"2026-04-15T18:38:43Z","timestamp":1776278323682,"version":"3.50.1"},"reference-count":34,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2025,8,20]],"date-time":"2025-08-20T00:00:00Z","timestamp":1755648000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100012547","name":"Natural Science Foundation of Guangxi Zhuang Autonomous Region","doi-asserted-by":"publisher","award":["2025GXNSFBA069014"],"award-info":[{"award-number":["2025GXNSFBA069014"]}],"id":[{"id":"10.13039\/100012547","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Phytotherapy Research"],"published-print":{"date-parts":[[2025,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>\n<jats:italic>Gynostemma pentaphyllum<\/jats:italic> (Thunb.), a traditional medicinal plant, is processed through different methods such as roller drying and shade drying. Shade\u2010dried <jats:italic>G. pentaphyllum<\/jats:italic> (SDGPs) contained monosaccharide and disaccharide saponins, while roller\u2010dried <jats:italic>G. pentaphyllum<\/jats:italic> (RDGPs) contained trisaccharide and tetrasaccharide saponins. These methods may affect its bioactive compounds, but their influence on non\u2010alcoholic fatty liver disease (NAFLD) remains unclear. This study aims to compare the anti\u2010NAFLD effects of <jats:italic>G. pentaphyllum<\/jats:italic> processed by these two methods and identify the active ingredients involved. Saponin fractions and monomers from both processing methods were isolated using chromatography. A mouse model of NAFLD was induced by a high\u2010fat and cholesterol diet to assess the therapeutic effects of the fractions. Molecular docking, surface plasmon resonance, and transcription analysis were used to evaluate the interactions of saponins with the Farnesoid X receptor (FXR). SDGPs exhibited better therapeutic effects on NAFLD in mice, with monosaccharide saponins being more effective than trisaccharide saponins. Gypenoside TN\u20101 outperformed Gypenoside XLVI in treating the NAFLD phenotype. Molecular analysis revealed a stronger binding affinity of monosaccharide saponins to FXR. SDGPs, especially the monosaccharide saponins, provide greater therapeutic benefits in treating NAFLD, likely through activation of the FXR\u2010FGF15 signaling pathway. These findings suggest that shade drying may be a more effective method for enhancing the therapeutic potential of <jats:italic>G. pentaphyllum<\/jats:italic> for NAFLD treatment.<\/jats:p>","DOI":"10.1002\/ptr.70049","type":"journal-article","created":{"date-parts":[[2025,8,20]],"date-time":"2025-08-20T15:09:17Z","timestamp":1755702557000},"page":"4428-4443","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Monosaccharide and Disaccharide Saponins of Shade\u2010Dried <i>Gynostemma Pentaphyllum<\/i> Confer Metabolic Benefits in Mice With Non\u2010Alcoholic Fatty Liver Disease"],"prefix":"10.1002","volume":"39","author":[{"given":"Fei","family":"Teng","sequence":"first","affiliation":[{"name":"Key Laboratory of Beijing for Quality Control of Chinese Medicine Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences  Beijing China"},{"name":"Shanghai Key Laboratory of Complex Prescription and MOE Key Laboratory for Standardization of Chinese Medicines Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haihui","family":"Liu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Beijing for Quality Control of Chinese Medicine Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yaping","family":"Jiang","sequence":"additional","affiliation":[{"name":"Shanghai Key Laboratory of Complex Prescription and MOE Key Laboratory for Standardization of Chinese Medicines Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuyang","family":"Xu","sequence":"additional","affiliation":[{"name":"Shanghai Key Laboratory of Complex Prescription and MOE Key Laboratory for Standardization of Chinese Medicines Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xunjiang","family":"Wang","sequence":"additional","affiliation":[{"name":"Shanghai Key Laboratory of Complex Prescription and MOE Key Laboratory for Standardization of Chinese Medicines Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xu","family":"Wang","sequence":"additional","affiliation":[{"name":"Shanghai Key Laboratory of Complex Prescription and MOE Key Laboratory for Standardization of Chinese Medicines Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ziqing","family":"Yang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Beijing for Quality Control of Chinese Medicine Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Min","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Beijing for Quality Control of Chinese Medicine Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rufeng","family":"Wang","sequence":"additional","affiliation":[{"name":"Shanghai Key Laboratory of Complex Prescription and MOE Key Laboratory for Standardization of Chinese Medicines Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhengtao","family":"Wang","sequence":"additional","affiliation":[{"name":"Shanghai Key Laboratory of Complex Prescription and MOE Key Laboratory for Standardization of Chinese Medicines Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li","family":"Yang","sequence":"additional","affiliation":[{"name":"Shanghai Key Laboratory of Complex Prescription and MOE Key Laboratory for Standardization of Chinese Medicines Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3329-487X","authenticated-orcid":false,"given":"Jingjing","family":"Zhu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Beijing for Quality Control of Chinese Medicine Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lili","family":"Ding","sequence":"additional","affiliation":[{"name":"Shanghai Key Laboratory of Complex Prescription and MOE Key Laboratory for Standardization of Chinese Medicines Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,8,20]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1089-8603(03)00032-6"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2021.06.012"},{"issue":"20","key":"e_1_2_12_4_1","first-page":"5314","article-title":"Simultaneous Quantitative Analysis of Nine Saponins in Gynostemma Pentaphyllum Before and After Heat Processing Based on 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