{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T19:43:58Z","timestamp":1791920638540,"version":"4.3.4"},"reference-count":58,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2025,10,7]],"date-time":"2025-10-07T00:00:00Z","timestamp":1759795200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,10,7]],"date-time":"2025-10-07T00:00:00Z","timestamp":1759795200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82273994"],"award-info":[{"award-number":["82273994"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82074084"],"award-info":[{"award-number":["82074084"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012247","name":"Program of Shanghai Academic Research Leader","doi-asserted-by":"publisher","award":["23XD1404000"],"award-info":[{"award-number":["23XD1404000"]}],"id":[{"id":"10.13039\/501100012247","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Phytotherapy Research"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Increasing evidence indicates that liver sinusoidal endothelial cells (LSECs) are crucial for non\u2010alcoholic steatohepatitis (NASH) development. Based on chlorogenic acid (CGA), which can improve NASH, this study aims to investigate the concrete mechanism of LSECs in NASH progression and identify CGA's key target in LSECs. High\u2010fat diet and methionine and choline deficient diet were used to induce NASH in mice. Western blot detecting endothelial molecules' expression, leukostasis assay, and Evans blue leakage were conducted to observe hepatic endothelial barrier dysfunction. Cell adhesion, transendothelial migration (TEM), and transendothelial electrical resistance were performed. Biotin pull\u2010down, cellular thermal shift assay, and surface plasmon resonance were used to identify CGA's key target. The pivotal roles of TGF\n                    <jats:italic>\u03b2<\/jats:italic>\n                    R1 and ID1 were investigated using adeno\u2010associated virus, plasmid, siRNA, or chemical inhibitor. CGA decreased ID1 expression by binding to TGF\n                    <jats:italic>\u03b2<\/jats:italic>\n                    R1 in LSECs, thereby reversing the change of hepatic vascular phenotype and abrogating hepatic infiltration of inflammatory cells during NASH progression. ID1 knockdown alleviated liver inflammation via suppressing TEM of inflammatory cells through reversing endothelial barrier dysfunction. However, TGF\n                    <jats:italic>\u03b2<\/jats:italic>\n                    R1 or ID1 overexpression reversed the CGA\u2010provided amelioration of liver inflammatory injury and endothelial barrier disorder. The decreased ID1 reduced NF\n                    <jats:italic>\u03ba<\/jats:italic>\n                    B\u2010initiated VCAM1 and ICAM1 expression and weakened ID1's inhibition on HIF1\n                    <jats:italic>\u03b1<\/jats:italic>\n                    \u2010mediated VE\u2010Cad expression, thereby maintaining hepatic endothelium homeostasis. LSECs are crucial for the progression of liver steatosis into NASH, and CGA alleviated liver inflammatory injury during NASH progression by inhibiting ID1\u2010initiated NF\n                    <jats:italic>\u03ba<\/jats:italic>\n                    B and HIF1\n                    <jats:italic>\u03b1<\/jats:italic>\n                    signaling pathways through binding to TGF\n                    <jats:italic>\u03b2<\/jats:italic>\n                    R1 in LSECs.\n                  <\/jats:p>","DOI":"10.1002\/ptr.70095","type":"journal-article","created":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T04:50:06Z","timestamp":1759899006000},"page":"5316-5344","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Chlorogenic Acid Alleviates Non\u2010Alcoholic Steatohepatitis via Suppressing Liver Inflammatory Injury by Targeting Transforming Growth Factor Beta Receptor\u20101 in Liver Sinusoidal Endothelial Cells"],"prefix":"10.1002","volume":"39","author":[{"given":"Hao","family":"Ouyang","sequence":"first","affiliation":[{"name":"The MOE Key Laboratory for Standardization of Chinese Medicines, Shanghai Key Laboratory of Compound Chinese Medicines and the SATCM Key Laboratory for New Resources and Quality Evaluation of Chinese Medicines Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine  Shanghai China"},{"name":"Department of Hepatology Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Miao","sequence":"additional","affiliation":[{"name":"The MOE Key Laboratory for Standardization of Chinese Medicines, Shanghai Key Laboratory of Compound Chinese Medicines and the SATCM Key Laboratory for New Resources and Quality Evaluation of Chinese Medicines Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine  Shanghai China"},{"name":"State Key Laboratory of Bioreactor Engineering East China University of Science and Technology  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong","family":"Zhang","sequence":"additional","affiliation":[{"name":"The MOE Key Laboratory for Standardization of Chinese Medicines, Shanghai Key Laboratory of Compound Chinese Medicines and the SATCM Key Laboratory for New Resources and Quality Evaluation of Chinese Medicines Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bin","family":"Lu","sequence":"additional","affiliation":[{"name":"The MOE Key Laboratory for Standardization of Chinese Medicines, Shanghai Key Laboratory of Compound Chinese Medicines and the SATCM Key Laboratory for New Resources and Quality Evaluation of Chinese Medicines Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinyu","family":"Zhang","sequence":"additional","affiliation":[{"name":"The MOE Key Laboratory for Standardization of Chinese Medicines, Shanghai Key Laboratory of Compound Chinese Medicines and the SATCM Key Laboratory for New Resources and Quality Evaluation of Chinese Medicines Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tianyu","family":"Zhang","sequence":"additional","affiliation":[{"name":"The MOE Key Laboratory for Standardization of Chinese Medicines, Shanghai Key Laboratory of Compound Chinese Medicines and the SATCM Key Laboratory for New Resources and Quality Evaluation of Chinese Medicines Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junfeng","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Hepatology Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhengtao","family":"Wang","sequence":"additional","affiliation":[{"name":"The MOE Key Laboratory for Standardization of Chinese Medicines, Shanghai Key Laboratory of Compound Chinese Medicines and the SATCM Key Laboratory for New Resources and Quality Evaluation of Chinese Medicines Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6159-2626","authenticated-orcid":false,"given":"Lili","family":"Ji","sequence":"additional","affiliation":[{"name":"The MOE Key Laboratory for Standardization of Chinese Medicines, Shanghai Key Laboratory of Compound Chinese Medicines and the SATCM Key Laboratory for New Resources and Quality Evaluation of Chinese Medicines Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,10,7]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0038-1666869"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccr.2010.10.023"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodres.2023.113513"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00418-009-0577-1"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1050-1738(01)00117-7"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrdp.2015.80"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu11112580"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0010(19990301)79:3<362::AID-JSFA256>3.0.CO;2-D"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2031337100"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jare.2023.02.007"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13075-016-0984-3"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.29466"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.32332"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI143690"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2021.724059"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12929-017-0357-5"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/133.6.1853"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41575-020-00411-3"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2019.10.028"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2019.02.012"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10753-016-0490-4"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40880-019-0427-z"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.20701"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.addr.2021.113869"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc3638"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/d41586\u2010024\u201000747\u20109"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20191548"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-04-1287"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/ki.2011.469"},{"issue":"1","key":"e_1_2_10_31_1","first-page":"49","article-title":"Regulation of Mammary Epithelial Cell Phenotypes by the Helix\u2010Loop\u2010Helix Protein, id\u20101","volume":"6","author":"Lin C. Q.","year":"1999","journal-title":"Endocrine"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-405943-6.00005-1"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0025-6196(24)00530-5"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12937-021-00694-5"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2017.12.019"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2015.05.011"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jnutbio.2022.109020"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/labinvest.2015.95"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodres.2021.110240"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.3337"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1002\/ptr.7401"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2018.01399"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-06-2643"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm.2017.36"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.31756"},{"issue":"4","key":"e_1_2_10_46_1","doi-asserted-by":"crossref","first-page":"218","DOI":"10.1038\/s41575-024-00909-0","article-title":"Resmetirom Proves Positive for NASH With Liver Fibrosis","volume":"21","author":"Ray K.","year":"2024","journal-title":"Nature Reviews Gastroenterology & Hepatology"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1572-0241.2004.40009.x"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms23052764"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2021.693048"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri3793"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.1017\/jns.2019.10"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.1002\/ptr.4751"},{"key":"e_1_2_10_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2022.102297"},{"key":"e_1_2_10_54_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.3c04419"},{"key":"e_1_2_10_55_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M608078200"},{"key":"e_1_2_10_56_1","doi-asserted-by":"publisher","DOI":"10.1097\/HEP.0000000000000004"},{"key":"e_1_2_10_57_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.28431"},{"key":"e_1_2_10_58_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11427-019-1596-4"},{"key":"e_1_2_10_59_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.201701536R"}],"container-title":["Phytotherapy Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ptr.70095","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/ptr.70095","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ptr.70095","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,31]],"date-time":"2026-01-31T11:05:49Z","timestamp":1769857549000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ptr.70095"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10,7]]},"references-count":58,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2025,11]]}},"alternative-id":["10.1002\/ptr.70095"],"URL":"https:\/\/doi.org\/10.1002\/ptr.70095","archive":["Portico"],"relation":{},"ISSN":["0951-418X","1099-1573"],"issn-type":[{"value":"0951-418X","type":"print"},{"value":"1099-1573","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,10,7]]},"assertion":[{"value":"2024-03-28","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-10-27","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-10-07","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}