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In this study, we investigated the potential lipid\u2010inhibitory and anti\u2010obesity effects of oligostilbene compounds derived from\n                    <jats:italic>Iris lactea<\/jats:italic>\n                    in 3T3\u2010L1 adipocytes. Particularly, Vitisin A on lipid accumulation in 3T3\u2010L1 adipocytes, as well as on lipid metabolism in HFD\u2010induced obese mice. The 3T3\u2010L1 adipocytes and HFD\u2010induced obese mice model were used in the study. In 3T3\u2010L1 adipocytes, MTT assay, oil red O staining, transmission electron microscopy, MitoSox\/DCFH\u2010DA probe, and western blotting were used to assess specific indicators. In HFD\u2010induced obese mice, lipid profile and hepatic injury were analyzed in the liver samples, histological sections were prepared and liver lipidomics analysis was performed, and western blotting was used to analyze the proteins associated with lipid lowering in Vitisin A. Vitisin A significantly reduced intracellular lipid accumulation, inhibited preadipocyte differentiation, and improved mitochondrial function in 3T3\u2010L1 adipocytes. Transcriptomics analysis revealed notable changes in adipogenic gene expression in Vitisin A\u2010treated 3T3\u2010L1 cells. Furthermore, Vitisin A administration in HFD\u2010induced obese mice resulted in smaller adipose depots and lower lipid concentrations. Lipidomics analysis indicated that Vitisin A modified lipid metabolic pattern in obese mice, significantly impacting triglycerols (TGs), phosphatidylcholines (PCs), and phosphatidylethanolamines (PEs). Mechanistic study revealed that Vitisin A is likely to activate the PPAR\u03b3\/PGC\u20101\u03b1 signaling pathway to ameliorating lipid metabolism disorders. The lipid\u2010inhibitory and anti\u2010obesity effects of Vitisin A from\n                    <jats:italic>Iris lactea<\/jats:italic>\n                    are regulated by lipid homeostasis and metabolism.\n                  <\/jats:p>","DOI":"10.1002\/ptr.70108","type":"journal-article","created":{"date-parts":[[2025,11,24]],"date-time":"2025-11-24T08:59:53Z","timestamp":1763974793000},"page":"286-303","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Vitisin A Regulates Lipid Metabolism in\n                    <scp>3T3<\/scp>\n                    \u2010\n                    <scp>L1<\/scp>\n                    Adipocytes and High\u2010Fat Diet\u2010Induced Obese Mice"],"prefix":"10.1002","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9466-585X","authenticated-orcid":false,"given":"Fangfang","family":"Tie","sequence":"first","affiliation":[{"name":"CAS Key Laboratory of Tibetan Medicine Research, 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Provincial Key Laboratory of Tibetan Medicine Research Northwest Institute of Plateau Biology, Chinese Academy of Sciences  Xining 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