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Cardamomin, a major constituent extracted from the stems and leaves of\n                    <jats:italic>Amomum villosum<\/jats:italic>\n                    , belongs to a chalcone with anti\u2010oxidative damage and neuroprotective activity. This study aimed to investigate the role and mechanism of cardamomin in oxidative damage and ischemic stroke. H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    stimulation model of BV\u20102 cells in\u00a0vitro and permanent middle cerebral artery occlusion model of rats were used. CCK\u20108 method, immunoblotting, immunofluorescence, comet assay, TTC staining, HE staining, and other methods were used to evaluate the protective effect of cardamomin on oxidative damage in\u00a0vivo and in\u00a0vitro. Our results demonstrated that cardamomin protected BV\u20102 cells from oxidative damage caused by H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    . Cardamomin inhibited oxidative stress by mediating NRF2 activation via the MEK\/ERK pathway. Cardamomin mitigated oxeiptosis by inhibiting the dephosphorylation of AIFM1 Ser116. Cardamomin resisted parthanatos by preventing the nuclear translocation of AIFM1 and the disruption of DNA. In addition, cardamomin attenuated ischemic stroke in rats. In summary, this study reveals the antioxidant damage mechanism and preventive effect on cerebral ischemic stroke of cardamomin.\n                  <\/jats:p>","DOI":"10.1002\/ptr.70114","type":"journal-article","created":{"date-parts":[[2025,10,14]],"date-time":"2025-10-14T04:52:22Z","timestamp":1760417542000},"page":"5378-5390","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Preprotective Effects of Cardamomin From\n                    <scp>\n                      <i>Amomum villosum<\/i>\n                    <\/scp>\n                    Lour. on\n                    <scp>\n                      H\n                      <sub>2<\/sub>\n                      O\n                      <sub>2<\/sub>\n                    <\/scp>\n                    \u2010Induced Oxidative Damage In\u00a0Vitro and Cerebral Ischemic Stroke In\u00a0Vivo"],"prefix":"10.1002","volume":"39","author":[{"given":"Man","family":"Zhu","sequence":"first","affiliation":[{"name":"Wuya College of Innovation, Key Laboratory of Structure\u2010Based Drug Design &amp; Discovery, Ministry of Education Shenyang Pharmaceutical University  Shenyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dejuan","family":"Sun","sequence":"additional","affiliation":[{"name":"Wuya College of Innovation, Key Laboratory of Structure\u2010Based Drug Design &amp; Discovery, Ministry of Education Shenyang Pharmaceutical University  Shenyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Wang","sequence":"additional","affiliation":[{"name":"Wuya College of Innovation, Key Laboratory of Structure\u2010Based Drug Design &amp; Discovery, Ministry of Education Shenyang Pharmaceutical University  Shenyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuxia","family":"Li","sequence":"additional","affiliation":[{"name":"Wuya College of Innovation, Key Laboratory of Structure\u2010Based Drug Design &amp; Discovery, Ministry of Education Shenyang Pharmaceutical University  Shenyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hua","family":"Li","sequence":"additional","affiliation":[{"name":"Wuya College of Innovation, Key Laboratory of Structure\u2010Based Drug Design &amp; Discovery, Ministry of Education Shenyang Pharmaceutical University  Shenyang China"},{"name":"Institute of Structural Pharmacology &amp; TCM Chemical Biology, College of Pharmacy Fujian University of Traditional Chinese Medicine  Fuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2196-1428","authenticated-orcid":false,"given":"Lixia","family":"Chen","sequence":"additional","affiliation":[{"name":"Wuya College of Innovation, Key Laboratory of Structure\u2010Based Drug Design &amp; Discovery, Ministry of Education Shenyang Pharmaceutical University  Shenyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,10,13]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.15212\/AMM\u20102021\u20100007"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1196\/annals.1427.014"},{"key":"e_1_2_12_4_1","first-page":"694","volume-title":"DNA Oxidation. 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